Pathological detection using immunohistochemistry(IHC)has become an indispensable process in the diagnosis confirmation of various cancers.However,the production of monoclonal antibodies is always very complex,expensive and time-consuming,and the batch differences are significant due to the corporeity and health statuses of animals may be different.In this work,an aptamer-based histochemistry(aptahistochemistry)assay was developed using a DNA aptamer for specific diagnosis of clinical breast cancer tissue sections.This aptahistochemistry assay can specifically distinguish Luminal A breast cancer molecular subtype from Luminal B(HER2+),HER2-enriched,and triple-negative breast cancer molecular subtypes,as well as para-carcinoma tissue,mastitis tissue and normal breast tissue.The accuracy of this aptahistochemistry assay for the diagnosis of Luminal A breast cancer was as high as 80％,which showed a great potential for clinical pathological diagnosis applications.