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选用有定时动情周期的非纯种雌性大鼠108只,分四组:Ⅰ组空气中汽油浓度为370±34mg/m~8,Ⅱ组空气中二氯乙烷浓度为15±3mg/m~8,Ⅲ组为汽油(310+_33mg/m~8)与二氯乙烷(11±1mg/m~8)混合染毒,Ⅳ组对照。连续4个月每周6天每天4小时染毒。染毒4个月后将雌鼠与健康雄鼠配偶6天(1只雄鼠配2只雌鼠)。以阴道涂片查见游动精子的当天为怀孕第一天。Ⅱ组的受孕雌鼠分出一部分从诊断妊娠起停止染毒,以阐明二氯乙烷是直接影响发育中胚胎或是在受孕前就通过性腺产生有害作用。除此以外,各组动物在整个妊娠期间均继续染毒。
A total of 108 non-pure female rats with regular estrous cycle were selected and divided into four groups: group Ⅰ gasoline concentration in air was 370 ± 34 mg / m ~ 8, group Ⅱ air dichloroethane concentration was 15 ± 3 mg / m ~ Groups 8 and 3 were mixed with gasoline (310 + _33 mg / m ~ 8) and dichloroethane (11 ± 1 mg / m ~ 8). 4 consecutive days 4 days a week for 6 days a week. After 4 months of exposure, female mice and healthy male spouses were mated for 6 days (1 male with 2 female mice). On the day of vaginal smear see swimming sperm on the first day of pregnancy. Part II of the group of pregnant females part of the suspension from the diagnosis of pregnancy, in order to elucidate that dichloroethane is directly affecting the developing embryo or in the gonadal adverse effect before conception. In addition, all groups of animals continued to be exposed throughout the pregnancy.