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目的:研究PPFP基因对人正常甲状腺细胞Nthy-ori 3-1生物学特性的影响,以明确PPFP是否具有致瘤作用。方法以我们前期实验构建的PPFP基因慢病毒载体稳定转染细胞株Nthy-ori 3-1PPFP、空白慢病毒载体稳定转染细胞株Nthy-ori 3-1Vector和未转染细胞株Nthy-ori 3-1为研究对象,以MTT法检测细胞增殖情况,平板克隆形成实验检测克隆形成数,软琼脂集落形成实验检测集落形成率,划痕愈合实验观察各组细胞体外迁徙运动能力,流式细胞仪检测各组细胞凋亡和细胞周期。结果以Nthy-ori 3-1Vector组和Nthy-ori 3-1组细胞为对照组,Nthy-ori 3-1PPFP组细胞较对照组在细胞增殖能力、平板克隆形成数、软琼脂集落形成率、体外迁徙运动能力均明显提高或增强,而细胞凋亡率明显下降,G0/G1期细胞明显减少,S期及G2/M期细胞则显著增加。结论PPFP基因可显著促进人正常甲状腺细胞Nthyori 3-1的增殖能力,并显著抑制其凋亡和促进其迁徙运动能力,提示该基因可能在滤泡状甲状腺癌恶性增殖和侵袭转移过程中起关键性作用。
OBJECTIVE: To study the effect of PPFP gene on the biological characteristics of Nthy-ori 3-1 in human normal thyroid cells so as to make clear whether PPFP has tumorigenicity. Methods The Nthy-ori 3-1PPFP cells stably transfected with PPFP gene lentiviral vector and the Nthy-ori 3-1 vector transfected by the lentiviral vector and the Nthy-ori 3- 1 as the research object, the cell proliferation was detected by MTT method, the number of colony formation was detected by plate clone formation assay, the colony formation rate was detected by soft agar colony formation assay, the migration ability of cells in each group was observed by scratch healing test, the flow cytometry Each group of apoptosis and cell cycle. Results Nthy-ori 3-1Vector group and Nthy-ori 3-1 group cells as control group, Nthy-ori 3-1PPFP group compared with the control group in cell proliferation, plate clone formation, soft agar colonization rate, in vitro Migration ability significantly increased or enhanced, while the apoptosis rate decreased significantly, G0 / G1 phase cells decreased significantly, S phase and G2 / M phase cells increased significantly. Conclusion PPFP gene can significantly promote the proliferation of normal thyroid cells Nthyori 3-1 and significantly inhibit the apoptosis and promote the migratory capacity, suggesting that the gene may play a key role in the malignant proliferation and invasion and metastasis of follicular thyroid carcinoma Sexual function.