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目的:探讨益气活血方和补肾生髓方对局灶性脑缺血再灌注损伤大鼠恢复期缺血半暗带脑源性神经营养因子(BDNF)和碱性成纤维细胞生长因子(bFGF)表达的影响。方法:采用线栓法阻塞大鼠左侧大脑中动脉,复制局灶性脑缺血再灌注模型,缺血2h分别再灌注1w、2w、3w,免疫组化SABC法分别检测缺血半暗带BDNF和bFGF的表达。结果:假手术组BDNF和bFGF弱阳性表达;再灌注各时间点,模型组BDNF和bFGF表达的平均吸收光密(OD值)升高,与假手术组比较有显著性差异(P<0.01,在3w时bFGF除外);在多个时间点益气活血方组、补肾生髓方组BDNF和bFGF表达的OD值均较模型组升高;两中药复方组间比较,在1w、2w,BDNF表达的OD值益气活血方组高于补肾生髓方组;第3w,补肾生髓方组高于益气活血方组,各时间点bFGF表达的OD值,两中药复方组间无显著差异。结论:益气活血方、补肾生髓方能通过促进缺血半暗带BDNF和bFGF的表达,抗脑缺血后损伤,有利于脑缺血后神经功能的恢复。
Objective: To investigate the effects of Yiqi Huoxue Decoction and Bushen Shengmi Recipe on brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) in the ischemic penumbra of rats with focal cerebral ischemia-reperfusion injury. ) The effect of expression. METHODS: The left middle cerebral artery was occluded by suture and the model of focal cerebral ischemia/reperfusion was duplicated. Ischemia was reperfusioned for 2h, 2w and 3w respectively. The immunohistochemical SABC method was used to detect the ischemic penumbra. Expression of BDNF and bFGF. Results: The expression of BDNF and bFGF was weakly positive in the sham operation group. The OD value of BDNF and bFGF expression in the model group increased at each time point of reperfusion, and there was a significant difference compared with the sham operation group (P<0.01). Except for bFGF at 3w), the OD values of BDNF and bFGF expression in Yiqi Huoxue Fang group and Bushen Shengmifang group at various time points were higher than those in the model group; between the two Chinese herbal compound groups at 1w and 2w, BDNF The expression of OD was increased in Yiqi Huoxue Decoction group than in Bushen Shengsui Decoction group; on the 3rd day, Bushen Sheng-Xue Decoction group was higher than Yiqi Huoxue decoction group, and the OD value of bFGF expression at each time point was not significantly different between the two Chinese herbal compound groups. . Conclusion: Yiqi Huoxue Decoction and Bushen Shengmai Decoction can promote the recovery of neurological function after cerebral ischemia by promoting the expression of BDNF and bFGF in the ischemic penumbra.