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目的:分别采用水蒸气蒸馏法(SD)和超临界CO2流体萃取法(SFE)提取滴鼻回医香药哈黑里里方中的挥发性成分,利用GC-MS进行鉴定分析,比较不同提取方法所得成分的差异。方法:GC-MS条件:Rxi-5Sil MS石英毛细管柱(30 m×0.25 mm,0.25μm);起始温度50℃,保留1 min,以10℃/min升温至120℃维持3 min,以3℃/min升温至200℃维持3 min,以5℃/min升温至290℃维持10 min至完成分析;载气为氦气;柱流量1.0 m L/min;分流比25∶1;进样口温度250℃;EI电离源70 e V;离子源温度230℃;扫描范围m/z 35~500。结果:SD法提取挥发油得率为0.21%,共鉴定出36个化合物,占挥发性成分总量的87.02%;SFE法提取挥发油得率为5.44%,共鉴定出38个化合物,占挥发性成分总量的97.47%。结论:采用SD法和SFE法提取得到的回医香药哈黑里里方挥发性成分有较大差异,为进一步阐明该方药的活性物质基础提供了实验数据。
OBJECTIVE: To extract the volatile constituents from the intranasal medicine Hehaoliri by steam distillation (SD) and supercritical CO2 fluid extraction (SFE), and identify the volatile components by GC-MS. Method differences in the resulting components. Methods: The GC-MS conditions were as follows: Rxi-5Sil MS quartz capillary column (30 m × 0.25 mm, 0.25 μm); initial temperature of 50 ° C for 1 min; heating to 120 ° C at 10 ° C / min for 3 min; ° C / min to 200 ° C for 3 min, ramp to 290 ° C at 5 ° C / min for 10 min to complete analysis; carrier gas was helium; column flow 1.0 m L / min; split ratio 25: Temperature 250 ℃; EI ionization source 70 e V; ion source temperature 230 ℃; scanning range m / z 35 ~ 500. Results: The yield of volatile oil extracted by SD method was 0.21%. A total of 36 compounds were identified, accounting for 87.02% of the total volatile components. The yield of volatile oil extracted by SFE method was 5.44%. A total of 38 compounds were identified, accounting for volatile components 97.47% of the total. CONCLUSION: The volatile components extracted by SD and SFE methods are different from each other. The experimental data are provided to further clarify the active substance basis of this prescription.