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目的 探讨核因子κB (NF κB)和细胞间粘附分子 1 (ICAM 1 )在吸烟大鼠气道上皮细胞中表达的变化及其在气道炎症形成过程中的作用。方法 Wistar大鼠 39只 ,随机分为不吸烟组、吸烟1个月组、3个月组 ,每组 1 3只。采用免疫组织化学染色和原位杂交技术半定量检测各组NF κB亚单位p65和ICAM 1的表达。结果 (1 )吸烟 1个月组、3个月组细支气管上皮细胞NF κB核染色阳性细胞百分比为 (63± 4) %、(51± 5) % ,与不吸烟组 [(2 7± 5) % ]比较 ,差异有显著性 (P分别 <0 .0 1 ) ;(2 )吸烟 1个月组、3个月组主、细支气管气道上皮细胞ICAM 1mRNA的表达为 0 .645± 0 .0 38、0 .747±0 0 4 1、0 .688± 0 .0 62、0 .80 9± 0 .0 2 3 ,与不吸烟组 (0 .52 6± 0 .0 2 3、0 .635± 0 .0 4 4 )比较差异有显著性 (P分别 <0 .0 1 ) ;而且 3个月组与 1个月组比较差异也有显著性 (P分别 <0 .0 5、0 .0 1 ) ;(3)吸烟 1个月组、3个月组主、细支气管气道上皮细胞ICAM 1蛋白表达为 0 .73± 0 .0 4、0 .94± 0 .0 5、0 .77± 0 .0 4、0 .99± 0 .0 3 ,与不吸烟组 (0 .57± 0 .0 4、0 .83± 0 .0 4 )比较 ,差异也有显著性 (P分别 <0 .0 1 ) ;而且 3个月组与 1个月组比较差异也有显著性 (P分别 <0 .0 5) ;(4)各组细支气管上皮
Objective To investigate the changes of NF κB and ICAM 1 in smoking rat airway epithelial cells and their roles in airway inflammation. Methods Thirty-nine Wistar rats were randomly divided into non-smoking group, smoking one month group and three-month group with 13 rats in each group. Immunohistochemical staining and in situ hybridization were used to semi-quantitatively detect the expression of NF-κB subunits p65 and ICAM-1. Results (1) The percentage of nuclear NF-κB positive cells in the bronchial epithelial cells in the smoking group was (63 ± 4)% and (51 ± 5)% respectively in the smoking group and the non-smoking group )%], The difference was significant (P <0.01). (2) The expression of ICAM 1mRNA in primary and bronchial airway epithelial cells of smoking 1 month group and 3 months group was 0.645 ± 0 .0 38,0 .747 ± 0 0 4 1,0 .688 ± 0 .0 62,0 .80 9 ± 0 .0 2 3, and non-smoking group (0 .52 6 ± 0 .0 2 3,0 .635 ± 0.04 4) (P <0 01). There was also a significant difference between the 3-month group and the 1-month group (P <0.05, respectively). 0 1). (3) ICAM 1 protein expression in primary and bronchial airway epithelial cells of 1-month smoking group was 0 .73 ± 0. 77 ± 0,04,90 ± 0,03, the difference was also significant (P <0, P <0, P <0 0) .0 1). There was also a significant difference between the 3-month group and the 1-month group (P <0.05, respectively). (4) The bronchial epithelium