均质酶免疫测定法(Homogeneous Enz-yme Immunoassay,HEI):是Rubenstin等(1972)创建的一种新型酶免疫测定技术。所谓“均质测定法”指的是在整个操作流程中,没有用理化方法将游离的酶标记物和已同相应抗原或抗体结合的酶标记物进行分离的步骤,仅将各种有关试剂加在一起,反应后就可直接测定结果。HEI可用全自动化仪器分析,重复性较好,也可减少传染性标本污染环境的机会,其敏感性因方法而异,部分方法已接近甚至超过常规的放免测定法(RIA)。测定结果时通常使用普通的分光光 Homogeneous Enzyme Immunoassay (HEI): A new enzyme immunoassay developed by Rubenstin et al. (1972). The term “homogeneous assay” refers to the step of isolating the free enzyme label from the enzyme label already bound to the corresponding antigen or antibody by physical and chemical methods throughout the entire process. Only the various reagents Together, the reaction can be directly measured results. HEI can be fully automated instrument analysis, better reproducibility, but also reduce the chances of infectious specimens of environmental pollution, the sensitivity varies with the method, some methods are close to or even beyond the conventional radioimmunoassay (RIA). The results of the determination of the ordinary use of ordinary light