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目的 对河北省卢龙县农村人口中儿童轮状病毒腹泻流行情况进行调查。方法 采用病毒核酸电泳和酶免疫方法对卢龙县 1998年轮状病毒腹泻发病季节采集的非菌性腹泻粪便标本进行轮状病毒检测。采用酶免疫和RT PCR方法对轮状病毒阳性标本进行血清型调查。结果 12 0份婴幼儿腹泻样本轮状病毒阳性率为 39 2 % ,病毒电泳型全为长型 ,血清分型发现G3型为流行优势株 ,占 61 7% ,其次为G1型占 36 2 % ,G4型占 6 4% ,轮状病毒腹泻病人最小年龄为 2月龄 ,最大为 2岁 ,男女之比为 1∶1 47。结论 卢龙县 1998年轮状病毒腹泻流行规律与全国情况基本一致 ,但流行毒株以G3型为主 ,与全国以G1型为主不同
Objective To investigate the epidemic situation of children ’s rotavirus diarrhea among rural population in Lulong County of Hebei Province. Methods The non-bacterial diarrhea stool samples collected in the season of rotavirus diarrhea in 1998 in Lulong county were detected by virus nucleic acid electrophoresis and enzyme immunoassay. Serotypes of rotavirus positive specimens were investigated by enzyme immunoassay and RT PCR. Results The positive rate of rotavirus in 12 infants and infants with diarrhea was 39.2% and the virus electrophoresis type was all long. Serotype G3 was predominant prevalence strain, accounting for 61.7%, followed by G1 type with 36.2% , G4 type accounted for 64%, rotavirus diarrhea patients minimum age of 2 months old, up to 2 years old, male to female ratio of 1:1 47. Conclusion The prevalence of rotavirus diarrhea in Lulong County in 1998 was basically consistent with that of the whole country, but the predominant strains were mainly G3,