We present a deep learning approach for living cells mitosis classification based on label-free quantitative phase imaging with transport of intensity equation methods.In the approach,we applied a pretrained deep convolutional neural network using transfer learning for binary classification of mitosis and non-mitosis.As a validation,we demonstrated the perfor-mances of the network trained by phase images and intensity images,respectively.The convolutional neural network trained by phase images achieved an average accuracy of 98.9％on the validation data,which outperforms the average accuracy 89.6％obtained by the network trained by intensity images.We believe that the quantitative phase microscopy in combination with deep learning enables researchers to predict the mitotic status of living cells noninvasively and efficiently.