论文部分内容阅读
采用复合区间作图法,对陆地棉sGK9708×0-153组合的重组近交系(RI)及永久F2群体在曲周(2009年)、安阳(2009,2010年)3个环境条件下的吐絮铃数和吐絮率进行QTL检测,共获得18个QTLs。其中,7个与吐絮铃数相关,加性效应在-0.46~0.33之间,可解释的表型变异为5.86%~11.28%;11个与吐絮铃率相关,加性效应在-3.64%~3.20%之间,可解释的表型变异4.68%~9.84%。这些QTLs主要分布在25号(吐絮铃数/吐絮率:3个/6个,下同)、16号(2个/2个)、18号(1个/2个)染色体和LG49(1个/1个)上。这18个QTLs中,qPOB-16-2在RI群体中的3个环境下被稳定检测到,qCOB-16-1、qPOB-25-3和qPOB-25-4在RI中的两个环境下被稳定检测到。这些QTLs可以应用于吐絮铃数和吐絮率的分子标记辅助选择。
The composite interval mapping method was used to investigate the effects of three combinations of upland cotton (sGK9708 × 0-153), recombinant inbred (RI) and permanent F2 populations on the three environmental conditions of Quzhou (2009) and Anyang (2009 and 2010) Boll number and boll opening rate QTL detection, a total of 18 QTLs. Among them, 7 were related to boll boll number, additive effect was between -0.46 and 0.33, and the explained phenotypic variation was 5.86% ~ 11.28%; 11 were related to boll boll rate, additive effect was -3.64% 3.20%, explainable phenotypic variation 4.68% ~ 9.84%. These QTLs were mainly distributed on the chromosomes 25 (1 boll / boll number: 3/6, the same below), 16 (2/2), 18 / 1). Of these 18 QTLs, qPOB-16-2 was stably detected in 3 of RI populations, and qCOB-16-1, qPOB-25-3 and qPOB-25-4 were both conserved in RI Stable detected. These QTLs can be applied to molecular marker-assisted selection of boll number and bolus rate.