论文部分内容阅读
为了探讨NO对碱胁迫下裸燕麦(Avena nuda)生理响应的调节作用,采用营养液培养,研究Ca~(2+)对外源NO供体硝普钠(SNP)所诱导的NaHCO_3胁迫下裸燕麦品种“定莜6号”幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明:25μmol·L-1SNP或6.5mmol·L-1 CaCl_2能够缓解75mmol·L-1 NaHCO_3胁迫对幼苗生长的抑制作用,叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和质膜H+-ATP酶活性较单独NaHCO3胁迫处理明显增强,O·-2、H_2O_2、丙二醛含量显著下降,抗坏血酸、可溶性糖、可溶性蛋白质、脯氨酸含量和K+/Na+显著提高;添加SNP的同时增添Ca~(2+)通道抑制剂~La(3+)或Ca~(2+)螯合剂EGTA抑制了SNP的这些作用。Ca~(2+)在NO诱导的碱胁迫下裸燕麦活性氧清除能力和渗透溶质含量提高、维持离子平衡中起重要作用,NO的作用可能依赖于胞浆Ca~(2+)的浓度。
In order to investigate the regulatory effect of nitric oxide (NO) on the physiological response of Avena nuda under alkaline stress, nutrient solution culture was used to study the effects of Ca 2+ on the expression of Naked Oats Variety “Ding No. 6 ” seedling growth, reactive oxygen metabolism and the impact of solute accumulation. The results showed that 25μmol·L-1 SNP or 6.5mmol·L-1 CaCl 2 could alleviate the inhibitory effect of 75 mmol·L -1 NaHCO 3 on the seedling growth. The activities of superoxide dismutase, catalase, peroxidase, ascorbate The activities of peroxidase and plasma membrane H + -ATPase were significantly increased compared with that of NaHCO3 alone. The contents of O · -2, H_2O_2 and MDA were significantly decreased. The contents of ascorbic acid, soluble sugar, soluble protein, proline and K + / Na + Significantly increased; addition of SNP while adding Ca 2+ channel inhibitor ~ La (3+) or Ca 2+ chelator EGTA inhibited these effects of SNP. Under the alkaline stress induced by NO, Ca 2+ promoted the activity of scavenging solute and the active oxygen scavenging ability and the content of osmotic solute, and played an important role in maintaining ion balance. The effect of NO on the content of Ca 2+ in cytoplasm may be dependent on the concentration of NO.