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目的 检测结肠正常上皮、息肉、腺瘤和腺癌细胞的凋亡。方法 应用原位缺口平移标记断裂DNA技术(ISNT)检测细胞凋亡。结果 ALI(凋亡标记指数)在正常上皮、息肉、腺瘤和腺癌分别为30.63±6.63％、34.38±7.35％、16.88±5.1％和2.38±2.51％,具有非常显著性差异。腺癌组按病理分级有显著差异,临床分期无意义。结论 在结肠上皮向腺瘤发展,继而向腺癌转化过程中存在明显的细胞凋亡的抑制,并可作为肿瘤分化程度的标志。
Objective To detect the apoptosis of colonic normal epithelium, polyps, adenomas and adenocarcinoma cells. Methods In situ nick translational translational fragmentation DNA (ISNT) was used to detect apoptosis. Results The ALI (apoptotic marker index) in normal epithelium, polyp, adenoma and adenocarcinoma were 30.63±6.63%, 34.38±7.35%, 16.88±5.1% and 2.38±2.51%, respectively. There was a very significant difference. The adenocarcinoma group had significant differences according to the pathological grade, and the clinical stage was not meaningful. Conclusion The development of adenoma in the colonic epithelium, followed by a significant inhibition of apoptosis in the process of adenocarcinoma transformation can be used as a marker of tumor differentiation.