目的:探讨应用时差成像(TLI)技术基于胚胎卵裂模式及时间参数进行胚胎挑选,对分裂期胚胎单胚胎移植(SET)妊娠结局的影响。方法:1将206个常规IVF周期患者胚胎行TLI动态观察,对其2 349枚非移植胚胎进行囊胚培养,回顾性分析胚胎发育的卵裂模式和时间参数,以囊胚质量为结局进行Logistic回归分析,建立优质胚胎筛选标准。2应用建立的优质胚胎筛选标准开展分裂期胚胎SET的前瞻性研究,用TLI观察88例常规IVF周期患者的胚胎发育情况,挑选优质胚胎进行SET,观察妊娠结局。结果:1分裂方式为正常卵裂、不均卵裂、非轴性卵裂的胚胎具有较高的优质囊胚形成率(分别为57.09%,44.44%及40.91%),时间参数从3-细胞分裂到4-细胞持续的时间(s2)(≤0.50 h)和发育至5-细胞的时间(t5)(≤48.93 h)对优质囊胚形成最具预测价值。2由此建立基于胚胎卵裂模式与时间参数的分层筛选模式,将其应用于分裂期胚胎SET,符合筛选条件的SET组临床妊娠率为73.24%(52/71)。结论:应用TLI,基于胚胎卵裂模式和时间参数建立了分层筛选模式,所挑选的优质胚胎可显著改善分裂期胚胎SET的妊娠结局。 Objective: To investigate the effect of time-lapse imaging (TLI) on pregnancy outcome of single embryo transfer (SET) of mitotic embryos based on embryo cleavage patterns and time parameters. METHODS: 1 Twenty-six routine IVF cycles of embryos were performed TLI dynamic observation. The blastocysts of 2 349 non-transplanted embryos were cultured. The cleavage pattern and time parameters of embryo development were retrospectively analyzed. Logistic Regression analysis, the establishment of high-quality embryo screening criteria. 2 Establishment of high-quality embryo screening criteria SET of schizotypal embryos prospective study of TLI was used to observe the routine IVF cycle in 88 cases of embryonic development, selection of high quality embryos SET, observation of pregnancy outcomes. Results: 1 blastomere with normal cleavage, non-uniform cleavage and non-axial cleavage had higher blastocyst formation rates (57.09%, 44.44% and 40.91%, respectively). The time parameters were from 3-cell The most predictive value of good blastocyst formation was the time to split to 4-cells (s2) (≤0.50 h) and the time to 5-cells (t5) (≤48.93 h). Thus, a stratified screening model based on embryonic cleavage pattern and time parameters was established and applied to SET of dividing embryos. The clinical pregnancy rate was 73.24% (52/71) in SET group that met the screening criteria. Conclusion: By using TLI, a stratified screening model was established based on embryonic cleavage patterns and time parameters. The selected high-quality embryos can significantly improve the pregnancy outcome of SET during mitosis.