论文部分内容阅读
巨噬细胞是体内重要的抗瘤效应细胞,使用巨噬细胞过继免疫治疗肿瘤已日益受到重视.本文用脂质体瘤苗免疫小鼠后,取其腹腔巨噬细胞(PEM)进行同系动物的过继免疫治疗,观察疗效.PEM来源于供体A.用H22肿瘤相关抗原脂质体瘤苗免疫的小鼠.供体B.用Frund’s adjuvant comleptH22癌细胞匀浆免疫的小鼠.供体C.艾氏腹水瘤肿瘤相关抗原脂质体瘤苗免疫的小鼠.供体D.用不含抗原的脂质体免疫的小鼠.供体E.用生理盐水代替免疫制剂免疫的小鼠.每只小鼠腹腔注射1×10~5个H22癌细胞后第二天腹腔分别转输各组PEM细胞5×10~6,对照组用生理盐水代替PEM.结果表明,死亡动物存活时间(X±SD)分别为A:32.7±9.3、B:30.6±6.8、C:31.2±9.2、D:31.1±6.6、
Macrophages are important anti-tumor effector cells in vivo. Adoptive immunotherapy of tumors using macrophages has received increasing attention. In this study, mice were immunized with liposomal tumor vaccines and their peritoneal macrophages (PEM) were taken for syngeneic animals. Adoptive immunotherapy for observation of efficacy. PEM was derived from donor A. Mice immunized with H22 tumor-associated antigen liposome vaccine. Donor B. Mice immunized with Frund’s adjuvant comleptH22 homogenate. Donor C. Ehrlich ascites tumor tumor-associated antigen Liposome vaccine immunized mice. Donor D. Mice immunized with liposome without antigen. Donor E. Mice immunized with physiological saline instead of immunizing agent. After intraperitoneal injection of 1×10~5 H22 cancer cells into the mice, each mouse received 5×10 6 PEM cells in the abdominal cavity. The control group received physiological saline instead of PEM. The results showed that the survival time of the dead animals (X± SD) are: A: 32.7±9.3, B: 30.6±6.8, C: 31.2±9.2, D: 31.1±6.6,