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阐明肝炎后肝纤维化发生机制。方法以ＨＢＶ基因组转染细胞和转基因小鼠为模型，应用原位杂交技术和斑点杂交技术，研究ＨＢＶ基因组的表达对Ⅰ、Ⅲ型胶原ｍＲＮＡ含量的影响。结果ＨＢＶ基因组的表达能显著提高转染细胞内Ⅰ，Ⅲ型胶原ｍＲＮＡ的水平。结论ＨＢＶ感染能促进Ⅰ．Ⅲ型胶原的转录，诱导肝纤维化的发生。
Clarify the mechanism of liver fibrosis after hepatitis. Methods The HBV genomic transfection cells and transgenic mice were used as the model. The in situ hybridization and dot blot hybridization were used to study the effect of HBV genome on the mRNA expression of type Ⅰ and type Ⅲ collagen. Results The expression of HBV genome could significantly increase the mRNA expression of type Ⅰ and type Ⅲ collagen in transfected cells. Conclusion HBV infection can promote Ⅰ. Type Ⅲ collagen transcription, induction of liver fibrosis.