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目的 检测食管癌组织中 18q11.2 - 12 .2区微卫星 DNA杂合性丢失情况 ,为定位新的抑癌基因提供依据。方法 应用聚合酶链反应 ,检测食管癌及癌旁组织中 D18S877、D18S5 35和 D18S9783个微卫星位点的杂合性丢失。结果 2 6例食管癌 D18S877、D18S5 35、D18S978位点的杂合性丢失频率分别为45 .0 %、38.9%和 6 0 .8% ,丢失频率最高的位点是 D18S978,定位于 18q12 .2。结论 18q12 .2区 D18S978位点杂合性丢失可能与食管癌的发生有关 ,该区域与脆性部位吻合 ,提示可能存在新的抑癌基因。
Objective To detect the loss of heterozygosity of microsatellite DNA in 18q11.2 - 12.2 region in esophageal cancer tissue and to provide basis for locating a new tumor suppressor gene. Methods Polymerase chain reaction (PCR) was used to detect the loss of heterozygosity for D18S877, D18S5 35 and D18S978 microsatellite loci in esophageal cancer and its adjacent tissues. Results The heterozygous loss frequencies of D18S877, D18S5 35 and D18S978 in 26 cases of esophageal cancer were 45.0%, 38.9% and 60.8%, respectively. The highest loss frequency was D18S978 and was located at 18q12.2 . Conclusion The loss of heterozygosity at site D18S978 in 18q12. 2 region may be related to the occurrence of esophageal cancer. This region agrees with the fragile site, suggesting that there may be a new tumor suppressor gene.