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沉积物中的叶绿素及其衍生物不易变化,是生产力的敏感指标。因此,根据其含量和种类可探查陆地生态系统的初级生产力。讨论了沉积物中叶绿素的提取及同步荧光测定叶绿素的方法,并应用于西双版纳热带雨林沉积物中叶绿素的测定,并对其环境意义进行了初步研究。研究发现,在最佳实验条件下,叶绿素a、b的浓度在0.5～200μg/L范围内与荧光信号呈良好的线性关系,相关系数分别为0.9994、0.9999;其检出限分别为1.410和0.376μg/L。本方法适合批量测定,为沉积物中低含量叶绿素的测定提供了一种简单而快速的测定方法。
Chlorophyll and its derivatives in the sediment is not easy to change, is a sensitive indicator of productivity. Therefore, the primary productivity of terrestrial ecosystems can be explored based on their content and type. The extraction of chlorophyll from sediment and the method of simultaneous determination of chlorophyll by fluorescence were discussed. The method was applied to the determination of chlorophyll in the sediments of tropical rainforest in Xishuangbanna, and its environmental significance was studied. The results showed that under the optimum conditions, the concentrations of chlorophyll a and b in the range of 0.5 ~ 200 μg / L showed a good linear relationship with the fluorescence signal, the correlation coefficients were 0.9994 and 0.9999, respectively. The detection limits were 1.410 and 0.376 μg / L. This method is suitable for batch determination and provides a simple and rapid method for the determination of low content of chlorophyll in sediments.