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重组链激酶（ｒ－ＳＫ）由工程菌提取液经ＳｅｐｈａｃｒｙｌＳ－２００柱纯化，比活性达１０５ＩＵ／ｍｇ，十二烷基硫酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）显示分子量为７８．０２４７×１０－２４ｋｇ的一条带。人新鲜血浆经Ｌｙｓｉｎｅ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析，ＳｅｐｈａｄｅｘＧ－２５凝胶过滤制备纤溶酶原（ｐｌｇ），比活性达２３．７ＩＵ／ｍｇ，活性回收率为１３．６％，ＳＤＳ－ＰＡＧＥ显示分子量为１４７．７４８９×１０－２４ｋｇ的一条带。对－茴香酸－对－脒基ｒ－ＳＫ和ｐｌｇ的混合物经苯酯盐酸盐（ＡＰＡＮ）修饰后制备茴香酰纤溶酶原链激酶激活剂复合物（ＡＰＳＡＣ），产物经Ｌ－ｌｙｓｉｎｅＳｅｐｈａｒｏｓｅ４Ｂ亲和层析，ＳＤＳ－ＰＡＧＥ显示分子量为７８．０２４７×１０－２４ｋｇ和１４７．７４８９×１０－２４ｋｇ两条带，其拟一级水解速率常数为１．２６×１０－４ｓｅｃ－１。体外溶栓实验证明其有溶栓活性。
Recombinant streptokinase (r-SK) was purified from Sephacryl S-200 column by the engineering bacteria extract with a specific activity of 105 IU / mg. The SDS-PAGE showed that the molecular weight of r-SK was 78. A band of 0247 × 10-24kg. Fresh plasma was purified by Lysine-Sepharose 4B affinity chromatography and Sephadex G-25 gel filtration to prepare plasminogen (plg) with a specific activity of 23.7 IU / mg and an activity recovery of 13.6%. SDS-PAGE showed that the molecular weight A band of 147.7489 × 10-24 kg. The mixture of p-anisic acid-p-amidino group r-SK and plg was modified with phenyl ester hydrochloride (APAN) to prepare an anisoylated plasminogen streptokinase activator complex (APSAC), and the product was purified by L-lysine Sepharose 4B affinity And chromatography. SDS-PAGE showed two bands with molecular weights of 78.0247 × 10-24kg and 147.7489 × 10-24kg, and their pseudo-first-order hydrolysis rate constants were 1.26 × 10-4sec-1. In vitro thrombolysis proved its thrombolytic activity.