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目的:研究五子衍宗丸对无精子症模型小鼠生精能力恢复作用的睾丸全基因表达谱。方法:利用白消安无精子症小鼠动物模型,给予五子衍宗丸灌胃给药78天(2个生精周期),以造模后自然恢复、溶媒和正常动物为3种对照,通过基因芯片技术进行数据分析。结果:五子衍宗丸对睾丸生精能力具有明显促进作用,在睾丸全基因表达谱中具有明显调节作用的基因中,差异表达2倍以上的基因842个(占2.65%,上调355个,下调487个),具有较强烈作用的差异表达4倍、5倍以上的上调基因和下调基因分别27个、9个和72个、23个,提示五子衍宗丸在促进生精过程中的基因表达以负向抑制/下调作用为主,以正向促进/上调作用为辅;主要通过对黏附功能、细胞周期、细胞因子-细胞因子受体作用、Hedgehog信号通路、Jak-Stat信号通路、Mapk信号通路、Notch信号通路、Toll样受体信号通路、Wnt信号通路等9个信号通路系统的干预调节实现生精能力提高的效果。结论:五子衍宗丸对无精子小鼠生精能力的恢复作用,主要是对生精干细胞的诱导分化和细胞增殖分裂相关基因的负向下调作用的调节并通过多途径实现的。
Objective: To study the testicular gene expression profile of Wuzi Yanzong Pill on the recovery of spermatogenesis in azoospermia model mice. Methods: The mice model of Busan Azoospermia was given intragastric administration of Wuzi Yanzong Pills for 78 days (two spermatogenic cycles) to recover spontaneously after modeling. Three kinds of control, vehicle and normal animal, Chip technology for data analysis. Results: Wuzi Yanzong Pills significantly enhanced testicular spermatogenic capacity. Among the genes with significant regulation in testicular gene expression profile, 842 (2.65%) were up-regulated more than 2 times ), With a strong role in the differential expression of 4 times, 5 times more than the up-regulated genes and down-regulated genes were 27, 9 and 72, 23, suggesting that Wuzi Yanzong Pill in promoting spermatogenesis in the process of gene expression to negative Mainly on the inhibition / down-regulation effect, supplemented by the positive promotion / up-regulation; mainly through the adhesion function, cell cycle, cytokine-cytokine receptor role, Hedgehog signaling pathway, Jak-Stat signaling pathway, Mapk signaling pathway, Notch signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway and other 9 signaling pathways to adjust the system to achieve sperm production ability to improve the effect. Conclusion: Wuzi Yanzong Pill can restrain the spermatogenesis of azoospermic mice mainly through the regulation of the down-regulation of spermatogenic stem cells and the down-regulation of the genes related to cell proliferation and division, and through multiple pathways.