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本实验用注射了17β-雌二醇(E_2)的母牛观察牛胎体分泌蛋白(CSP)对子宫产生前列腺素(PG)F_(2α)的作用,目的在于确定注射E_2后,CSP是否可减少子宫产生PGF_(2α)。实验用38只交配后第17或18天的母牛子宫,用37℃无菌生理盐水冲洗2次,收集含胎体的盐水,并把胎体转移到无菌培养基上,孵育30小时。然后离心、取上清液透析、超滤,制得CSP溶液。由孕18天的牛体得到血清蛋白质。将10只乳牛随机分为对照组(n=5)和实验组(n=5)。从动情后期的第12天起,每天收集颈静脉血样。在动情后
In this study, we observed the effect of CSP on the production of prostaglandin (PG) F_ (2α) in uterus by injecting 17β-estradiol (E_2) into the uterus. The aim was to determine if CSP Reduces uterine production of PGF_ (2α). Experiments were performed on 38 cows on the 17th or 18th day after mating with rhesus twice in 37 ° C sterile saline. The carcass-containing saline was collected and the carcasses were transferred to sterile medium and incubated for 30 hours. Then centrifuged, the supernatant was dialyzed, ultrafiltrated to prepare CSP solution. Serum protein is obtained from the pregnant cow for 18 days. Ten cows were randomly divided into control group (n = 5) and experimental group (n = 5). From day 12 of late estrus, jugular venous blood samples were collected daily. After the passion