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目的探讨在电刺激干预下大鼠脊髓损伤节段巢蛋白(Nestin)和胶质纤维酸性蛋白(glial fibrilary acidicprotein,GFAP)的表达及二者关系与非电刺激组的区别,及其产生的可能原因。方法应用Allen′s法建立大鼠脊髓损伤模型,电刺激组于术后24 h开始给予夹脊穴和足三里穴电刺激,频率10 Hz,电压2.5 V,每天1次,每次30 min,空白组和对照组无特殊处理。采用BBB评分观察大鼠后肢运动情况,应用免疫组织化学染色图像处理系统分析和显示脊髓损伤后不同时期(1、3、5、7、14 d)脊髓T9巢蛋白和GFAP表达变化。结果行为学观察,电刺激组大鼠术后1周BBB评分显著高于对照组(P<0.05),低于空白组(P<0.05)。电刺激组1~7 d巢蛋白表达显著升高(P<0.05),第7天达到峰值并且其表达明显高于对照组(P<0.05),第14d巢蛋白表达降低,但同比高于对照组。电刺激组1~5 d GFAP表达升高,第5天达到峰值,7~14 dGFAP表达显著下降(P<0.05)。结论电刺激干预下,脊髓组织局部巢蛋白表达增强,GFAP持续表达被抑制,电刺激促进损伤脊髓神经元再生修复和功能恢复,并抑制星形胶质细胞持续反应性增生,减少胶质瘢痕形成。
Objective To investigate the expression of Nestin and glial fibrillary acidic protein (GFAP) in spinal cord injury-induced spinal cord injury and the possible relationship between them and non-electrical stimulation the reason. Methods The rat spinal cord injury model was established by Allen’s method. Electro-stimulation was given to the Jiaji acupoint and the Zusanli acupoint at 24 hours after the operation, with frequency of 10 Hz and voltage of 2.5 V, once a day for 30 min, Blank group and control group without special treatment. The BBB score was used to observe the hindlimb movement in rats. The expressions of T9 nestin and GFAP in the spinal cord at different time points (1, 3, 5, 7 and 14 days after SCI) were analyzed by immunohistochemical staining and image processing system. Results After behavioral observation, the BBB score of rats in electrical stimulation group was significantly higher than that in control group (P <0.05) and lower than that in blank group (P <0.05). The expression of nestin increased from day 7 to day 7 in electrical stimulation group (P <0.05), peaked on day 7, and was significantly higher than that in control group (P <0.05). Nestin expression decreased on the 14th day but was higher than the control group. The expression of GFAP was increased from 1 to 5 days after electrical stimulation in rats, reaching the peak on the 5th day and decreasing significantly on the 7th to the 14th day (P <0.05). Conclusion Under the stimulation of electrical stimulation, the expression of local nestin in spinal cord tissue is enhanced, and the sustained expression of GFAP is inhibited. Electrical stimulation can promote the regeneration and repair of injured spinal cord neurons and inhibit the persistent reactive hyperplasia of astrocytes and reduce the glial scar formation .