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目的检测结直肠癌患者粪便和血清的M2型丙酮酸激酶(M2-PK)、APC、K-ras水平,并分析M2-PK、APC、K-ras的水平与结直肠癌病理T分期的关系。方法将2015年1-10月在该院手术治疗的结直肠癌患者200例纳入该研究作为患者组,另外选取健康体检者100例作为对照组。采用酶联免疫吸附测定(ELISA)检测结直肠癌患者血清和粪便的M2-PK、APC、Ras水平,并结合患者的病理资料对M2-PK、APC、K-ras的水平与结直肠癌病理T分期的关系进行分析。结果结直肠癌患者血清和粪便的M2-PK、APC、K-ras的表达水平分别高于对照组(P<0.05)。M2-PK、APC、K-ras的表达与结直肠癌的病理T分期有密切关系,且临床病理T分期较晚的患者M2-PK、APC、K-ras的表达水平更高(P<0.05)。结论结直肠癌患者血清和粪便的M2-PK、APC、K-ras水平较高且与肿瘤的临床病理T分期有关。M2-PK、APC、K-ras可作为早期诊断结直肠癌的分子标志物。
Objective To detect the levels of M2-PK, APC and K-ras in excrement and serum of patients with colorectal cancer and to analyze the relationship between the levels of M2-PK, APC and K-ras and the pathological T stage of colorectal cancer . Methods A total of 200 patients with colorectal cancer undergoing surgery in the hospital from January to October 2015 were included in the study. 100 healthy people were selected as the control group. The levels of M2-PK, APC and Ras in serum and feces of patients with colorectal cancer were detected by enzyme-linked immunosorbent assay (ELISA). The levels of M2-PK, APC and K-ras in patients with colorectal cancer were analyzed. T staging of the relationship analysis. Results The levels of M2-PK, APC and K-ras in serum and feces of patients with colorectal cancer were significantly higher than those of the control group (P <0.05). The expression of M2-PK, APC and K-ras were closely related to the pathological T stage of colorectal cancer, and the expression of M2-PK, APC and K-ras were higher in patients with later stage of clinical pathology (P <0.05 ). Conclusions The levels of M2-PK, APC and K-ras in colorectal cancer patients and their stools are higher than those in the clinical stage. M2-PK, APC, K-ras can be used as molecular markers for the early diagnosis of colorectal cancer.