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目的检测兔动脉粥样斑块中趋化因子受体4(chemokine receptor4,CXCR4)蛋白表达与血管内皮生长因子(vascular endothelial growth factor receptor,VEGF)mRNA表达变化及其相关性分析,探讨CXCR4在粥样斑块血管生成中的作用及可能机制。方法 15只新西兰大白兔高脂饲料喂养+球囊拉伤腹主动脉建立动脉粥样硬化模型,分别于4、8、12周末处死动物,截取动脉血管,采用Western blot法检测CXCR4蛋白含量变化;实时荧光定量PCR检测VEGF mRNA表达变化,对两者结果进行相关性统计学分析;免疫组织化学法检测动脉粥样硬化斑块CXCR4、CD34表达与定位。结果第4、8、12周3个时相点动脉血管CXCR4蛋白含量分别为(0.46±0.03)、(0.58±0.05)、(0.65±0.07),VEGFmRNA表达分别为(1.04±0.24)、(1.99±0.21)、(3.97±0.75),两者之间呈显著正相关(Pearson相关系数r=0.869,P<0.05)。免疫组织化学CXCR4染色在血管内膜及斑块内部可见多处阳性染色;CD34染色在血管内膜斑块部位可见丰富的新生血管,棕黄色阳性表达。结论兔腹主动脉粥样硬化进程中,CXCR4和VEGF表达含量呈正相关,可能在动脉粥样硬化斑块的血管生成中具有重要作用。
Objective To detect the expression of chemokine receptor 4 (CXCR4) protein and the expression of vascular endothelial growth factor receptor (VEGF) in atherosclerotic plaque in rabbits and to explore the correlation between the expression of CXCR4 and porcine heart disease Role of plaque angiogenesis and its possible mechanism. Methods Fifty New Zealand white rabbits were fed with high-fat diet and balloon-injured abdominal aorta. Atherosclerosis models were established at the end of 4, 8 and 12 weeks respectively. Arteries were taken and the content of CXCR4 protein was detected by Western blot. The expression of VEGF mRNA was detected by real-time fluorescence quantitative PCR, and the correlation between them was statistically analyzed. The expression and location of CXCR4 and CD34 in atherosclerotic plaque were detected by immunohistochemistry. Results The levels of CXCR4 protein in arterial blood were (0.46 ± 0.03), (0.58 ± 0.05) and (0.65 ± 0.07), respectively at the 4th, 8th and 12th week. The expressions of VEGF mRNA were (1.04 ± 0.24) and ± 0.21) and (3.97 ± 0.75), respectively (Pearson correlation coefficient r = 0.869, P <0.05). Immunohistochemical CXCR4 staining in the intima and plaque within the visible multiple positive staining; CD34 staining in the intima plaque can be seen rich in neovascular, brown-positive expression. Conclusion The expression of CXCR4 and VEGF in rabbit abdominal aorta during atherosclerosis is positively correlated, which may play an important role in angiogenesis of atherosclerotic plaque.