目的建立一种简单的小鼠部分睡眠剥夺(partial sleep deprivation,PSD)模型,并对该模型进行评价。方法将48只小鼠随机分成2批,每批24只,每批小鼠再随机分成对照组和PSD模型组,每组12只。利用小鼠入睡时肌肉松弛,不能站立入睡的生理特征,采用水上站立法,将PSD模型组小鼠放置在0.6 cm水深的饲养笼中进行部分睡眠剥夺,剥夺强度为18 h/d,连续剥夺2 d。观察睡眠剥夺对小鼠体重、自主活动、大脑单胺类神经递质(NE、DA和5-HT)含量的影响。结果睡眠剥夺前后PSD模型组小鼠平均体重分别为(23.03±1.23)、(20.82±1.33)g,体重明显减轻(P<0.01);睡眠剥夺前对照组和PSD模型组小鼠自主活动次数分别为(58.2±24.3)、(50.0±14.5)次,睡眠剥夺后,2组小鼠的自主活动次数分别为(51.8±18.2)、(9.1±7.2)次,PSD模型组小鼠自主活动次数明显减少(P<0.01)。睡眠剥夺后,对照组小鼠大脑NE、DA和5-HT含量分别为(44.47±4.84)、(210.54±21.47)、(120.79±14.77)ng/g脑湿重;PSD模型组小鼠大脑NE、DA和5-HT含量分别为(37.61±3.57)、(168.23±25.09)、(97.57±10.48)ng/g脑湿重,与对照组相比,均明显降低(均P<0.01)。结论成功建立水上站立法复制小鼠部分睡眠剥夺模型。该方法可致小鼠大脑NE、DA、5-HT分泌失衡,增加小鼠的抑制性,使小鼠疲劳、睡眠-觉醒过程紊乱,能较好地模拟部分睡眠剥夺人群生理状态。且该模型简单经济,无需特制实验材料和设备,可同时对多个小鼠进行部分睡眠剥夺,适用于抵抗睡觉剥夺不良影响的实验研究。 Objective To establish a simple model of partial sleep deprivation (PSD) in mice and evaluate the model. Methods 48 mice were randomly divided into 2 batches of 24 batches. Each group of mice were randomly divided into control group and PSD model group, 12 in each group. Mice were used to sleep when they were asleep and could not stand the physiological characteristics of falling asleep. The PSD model mice were placed in a feeding cage at a depth of 0.6 cm for partial sleep deprivation, with a deprivation strength of 18 h / d and continuous deprivation 2 d. To observe the effects of sleep deprivation on the body weight, autonomic activity and the levels of monoamine neurotransmitters (NE, DA and 5-HT) in mice. Results Before and after sleep deprivation, the mean body weights of mice in PSD model group were (23.03 ± 1.23) and (20.82 ± 1.33) g, respectively, and the body weight was significantly reduced (P <0.01). The number of spontaneous activity of PSD model control group and PSD model group (58.2 ± 24.3) and (50.0 ± 14.5) times, respectively. After sleep deprivation, the frequency of spontaneous activity in the two groups of mice was (51.8 ± 18.2) and (9.1 ± 7.2) times, respectively. Decrease (P <0.01). After sleep deprivation, the contents of NE, DA and 5-HT in the control group were (44.47 ± 4.84), (210.54 ± 21.47) and (120.79 ± 14.77) ng / g, respectively. Brain PSD (37.61 ± 3.57), (168.23 ± 25.09) and (97.57 ± 10.48) ng / g brain wet weight respectively, which were significantly lower than those in the control group (all P <0.01). Conclusion The successful establishment of a waterborne station replicates mouse partial sleep deprivation model. This method can cause imbalance of NE, DA and 5-HT secretion in mouse brain and increase the inhibitory effect of mice. It can make the mouse sleep disorder and disorder of sleep-wake process, and can better simulate the physiological status of partial sleep deprivation. Moreover, the model is simple and economical, and does not require special experimental materials and equipment. It can perform partial sleep deprivation on multiple mice at the same time and is suitable for experimental study on the adverse effects of sleep deprivation.