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目的:探讨江苏汉族人群脂联素通路基因多态性和环境的联合作用与2型糖尿病的关系。方法:采用病例对照研究,收集新诊断T2DM病例(1105例)和健康对照(1107例)的临床和流行病学相关资料。利用Real-time PCR联合Taqman-MGB探针方法对19个多态性位点进行基因分型。结果:显性遗传模型的单因素Logistic回归分析发现ADIPOQ基因rs7649121、PPARγrs3856806基因型频率在病例与对照组的分布差异有统计学意义(P<0.05)。多因素Logistic回归分析显示糖尿病家族史、高血压史、腰围、血清脂联素浓度、吸烟、饮酒、ADIPOQ rs7649121、rs12495941,PPARγrs3856806等因素与T2DM的发病风险存在统计学关联(P<0.05)。结论:脂联素通路多态性与环境联合作用对于T2DM遗传易感性可能具有一定的影响。
Objective: To investigate the association between adiponectin pathway gene polymorphism and environment and the type 2 diabetes mellitus in Jiangsu Han population. Methods: A case-control study was conducted to collect clinical and epidemiological data on newly diagnosed T2DM cases (1105 cases) and healthy controls (1107 cases). Nineteen polymorphic sites were genotyped using Real-time PCR combined with Taqman-MGB probe. Results: The single factor Logistic regression analysis of dominant genetic model found that ADIPOQ gene rs7649121, PPARγ rs3856806 genotype frequencies in the case and the control group distribution was statistically significant (P <0.05). Multivariate logistic regression analysis showed that family history of diabetes, history of hypertension, waist circumference, serum adiponectin concentration, smoking, drinking, ADIPOQ rs7649121, rs12495941, PPARγrs3856806 and other factors were associated with the risk of T2DM (P <0.05). Conclusion: The combination of adiponectin pathway and the environment may have a certain impact on genetic susceptibility to T2DM.