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目的观察岷当归对高脂饲料(HDF)联合链脲佐菌素(STZ)诱导的Ⅱ型糖尿病小鼠血清白细胞介素-6(IL-6)、白细胞介素-22(IL-22)和肿瘤坏死因子-α(TNF-α)含量的影响。方法将40只昆明种小鼠随机分为空白组、模型组、防治组和治疗组,每组10只。防治组提前2周灌胃当归水煎液,2周后除空白组外,其他组采用HDF联合STZ腹腔注射造模,造模成功后防治组和治疗组灌胃当归水煎液,其他2组继续灌胃生理盐水,连续灌胃4周。在处死小鼠前1天,各组小鼠进行葡萄糖耐量(OGTT)试验,经药物干预后采用酶联免疫法检测各组小鼠血清IL-6,IL-22和TNF-α的含量。结果与模型组比较,防治组和治疗组小鼠OGTT值,血清IL-6,IL-22和TNF-α含量均降低,差异均有统计意义(P<0.05);且防治组小鼠OGTT值,血清IL-6,IL-22和TNF-α含量下降更明显,差异均有统计意义(P<0.05)。结论岷当归可以调节HDF联合STZ诱导的Ⅱ型糖尿病小鼠血清IL-6,IL-22和TNF-α的分泌,缓解Ⅱ型糖尿病小鼠的炎症反应,从而起到调节Ⅱ型糖尿病小鼠免疫平衡的作用。
Objective To observe the effects of Min Danggui on the levels of serum interleukin-6 (IL-6), interleukin-22 (IL-22), and interleukin-22 in type 2 diabetic mice induced by high fat diet (HDF) and streptozotocin (STZ) Tumor necrosis factor-α (TNF-α) content. Methods Forty Kunming mice were randomly divided into blank group, model group, prevention group and treatment group, with 10 mice in each group. Control group 2 weeks in advance angelica decoction, 2 weeks after the addition of the blank group, other groups using HDF combined with STZ intraperitoneal injection modeling, control group and the treatment group after successful model was drenched Angelica decoction, the other two groups Continue to intragastric administration of saline, continuous gavage for 4 weeks. One day before the mice were sacrificed, the mice in each group were subjected to glucose tolerance test (OGTT). The levels of IL-6, IL-22 and TNF-α in sera of mice were detected by enzyme-linked immunosorbent assay after drug intervention. Results Compared with the model group, the levels of OGTT, IL-6, IL-22 and TNF-α in the control group and the treatment group were decreased, the differences were statistically significant (P <0.05) , The levels of serum IL-6, IL-22 and TNF-αincreased more obviously, the differences were statistically significant (P <0.05). Conclusion Min Angelica can regulate the secretion of IL-6, IL-22 and TNF-α in type 2 diabetic mice induced by HDF combined with STZ and relieve the inflammatory reaction in type 2 diabetic mice and thus regulate the immunity of type 2 diabetic mice Balanced effect.