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目的:比较低密度脂蛋白-阿克拉霉素(LDL-ACM)复合物和游离ACM对胃癌细胞株SGC-7901的抑制作用,从而为以LDL为载体介导的癌瘤靶向治疗提供理论支持。方法:37℃、CO25%及饱和湿度培养箱培养人上皮样胃癌细胞株SGC-7901及取自胃壁经原代培养获得的胃成纤维细胞。以LDL作为脂溶性抗肿瘤药物ACM的载体,温育交换法制备LDL-ACM复合物。观察LDL-ACM复合物及游离ACM对胃癌细胞株SGC-7901及胃成纤维细胞的增殖、细胞DNA合成及蛋白质合成的抑制作用,并进行统计学分析。结果:LDL-ACM复合物对胃癌细胞株SGC-7901的生长、DNA及蛋白质合成的抑制作用均显著强于游离ACM,且差异有统计学意义(P分别<0.01、0.05、0.005),且在药物浓度为1.2500～5.0000μg/mL范围内对于细胞DNA合成的抑制作用更显著(P<0.05)。而LDL-ACM复合物和游离ACM对正常胃成纤维细胞的生长、DNA的合成及蛋白质合成的抑制作用差异无统计学意义(P>0.05)。结论:以LDL为载体介导的肿瘤药物的细胞抑制作用强于游离抗肿瘤药物。
OBJECTIVE: To compare the inhibitory effect of LDL-ACM complex and free ACM on gastric cancer cell line SGC-7901 and to provide theoretical support for the targeted therapy of cancer targeting with LDL . Methods: Human epithelial gastric cancer cell line SGC-7901 and gastric fibroblast derived from the stomach wall were cultured in 37 ℃, CO25% and saturated humidity incubator. LDL as a lipophilic anticancer drug ACM carrier, incubation exchange method preparation LDL-ACM complex. The inhibitory effects of LDL-ACM complex and free ACM on the proliferation, cell DNA synthesis and protein synthesis of gastric cancer cell lines SGC-7901 and gastric fibroblasts were observed and statistically analyzed. Results: The inhibitory effect of LDL-ACM complex on the growth, DNA and protein synthesis of gastric cancer cell line SGC-7901 was significantly stronger than that of free ACM, and the difference was statistically significant (P <0.01,0.05,0.005) The inhibitory effect on the DNA synthesis was more significant (P <0.05) when the drug concentration ranged from 1.2500 to 5.0000 μg / mL. However, LDL-ACM complex and free ACM had no significant difference on the growth of normal gastric fibroblasts, DNA synthesis and protein synthesis (P> 0.05). Conclusion: The cytotoxicity of tumor drugs mediated by LDL is stronger than that of free antitumor drugs.