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2012年,江苏南京菜豆上出现了一种新的病毒病害,病株表现明显的叶片皱缩、植株矮化等症状。根据其症状及介体发生状况,对其伴随的病毒种类进行了研究,结果从中检测到一种粉虱传双生病毒,对其基因组DNA-A组分克隆测序后发现其全长2 781 bp,编码6个ORF,BLAST及聚类分析结果显示该病毒与番茄黄化曲叶病毒同源性最高(99%),是番茄黄化曲叶病毒的一个分离物。这是江苏省番茄黄化曲叶病毒侵染菜豆的首次报道,暗示番茄黄化曲叶病毒可能是我国菜豆种植的重要潜在威胁。
In 2012, there was a new virus disease on the beans in Nanjing, Jiangsu Province. The diseased plants showed obvious symptoms of leaf shrinkage and plant dwarfing. According to its symptoms and the status of the mediator, the virus species that accompany it were studied. As a result, a whitefly-transmitted geminivirus was detected. The full-length genomic DNA-A component was found to be 2 781 bp in length after cloning and sequencing. The result of BLAST and cluster analysis showed that the virus had the highest homology (99%) with Tomato yellow leaf curl mosaic virus and was an isolate of Tomato yellow leaf curl virus. This is the first report of tomato leaf curl mosaic virus infection in Jiangsu province, suggesting that tomato yellow curl leaf virus may be an important potential threat to bean cultivation in China.