Aim:The spinal cord is pivotal in immobility induced by volatile anestheticsbecause the anesthetics depress the activity of motor neurons in the spinal cord.The aim of this study was to observe the effects of sevoflurane on pain process-ing at the spinal level.Methods:The firing of the gastrocnemius muscle wasevoked by electrical stimulation to the ipsilateral hindpaw in rats.The nociceptiveC response of electromyography(EMG)was selected to study.The GABAAreceptor antagonist bicuculline(0.1 mg/kg)and opioid receptor antagonist nalo-xone(0.4 mg/kg)were administered intravenously,either in the presence or in theabsence of 1.0% sevoflurane.Results:In rats with transected spinal cord,sevoflurane produced a profound reduction in the C response in a dose-and time-dependent manner.In the presence of 1.0% sevoflurane,the C responses wereincreased after injections of bicuculline and naloxone.Conclusion:Sevofluraneis a volatile anesthetic that acts directly on the spinal cord to suppress the nocice-ptive reflex.The sevoflurane-induced suppression of the C response is antago-nized by either bicuculline or naloxone.The results suggest that spinal GABA_Areceptors and opioid peptide receptors are involved in the sevoflurane-inducedsuppression of spinal nociception. Aim: The spinal cord is pivotal in immobility induced by volatile anestheticsbecause the anesthetics depress the activity of motor neurons in the spinal cord. The aim of this study was to observe the effects of sevoflurane on pain process-ing at the spinal level. Methods: The firing of the gastrocnemius muscle wasevoked by electrical stimulation to the ipsilateral hindpaw in rats. Nociceptive C response of electromyography (EMG) was selected to study. GABAA receptor antagonist bicuculline (0.1 mg / kg) and opioid receptor antagonist nalo-xone / kg) were administered intravenously, either in the presence or in the presence of the body of 1.0% sevoflurane. Results: In rats with transected spinal cord, sevoflurane produced a profound reduction in the C response in a dose-and time-dependent manner. In the presence of 1.0% sevoflurane, the C responses were built after injections of bicuculline and naloxone. Conflusion: Sevoflurane a a volatile anesthetic that acts directly on the spinal cord to suppress the noc ice-ptive reflex. The sevoflurane-induced suppression of the C response is antago-nized by either bicuculline or naloxone. The results suggest that spinal GABA_A receptors and opioid peptide receptors are involved in the sevoflurane-induced compression of spinal nociception.