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本发明公开了一种基于纳米颗粒和化学发光的核酸适体传感器,以及其制备方法和应用。以Fe3O4Si O2磁性纳米颗粒和双功能化金纳米颗粒作为固相载体,活化的羧基化Fe3O4Si O2磁性纳米颗粒与氨基修饰的核酸适体结合形成磁性纳米颗粒-核酸适体复合物,再与双功能化金纳米颗粒杂交形成磁性纳米颗粒-核酸适体-双功能化金纳米颗粒复合物,最后与链霉亲和素标记的碱性磷酸酶结合,得到所述传感器。该核酸适体传感器具有磁性便于快速磁分离,又具有化学发光法快速、高效检测的优点,在生物医学检测方面具有很大的应用价值。
The present invention discloses a nucleic acid aptamer sensor based on nanoparticles and chemiluminescence and a preparation method and application thereof. Using Fe3O4SiO2 magnetic nanoparticles and bifunctional gold nanoparticles as solid carriers, the activated carboxylated Fe3O4SiO2 magnetic nanoparticles combine with the amino-modified aptamer to form a magnetic nanoparticle-aptamer complex and then react with bifunctional The gold nanoparticles are hybridized to form a magnetic nanoparticle-aptamer-bifunctional gold nanoparticle complex, and finally the streptavidin-labeled alkaline phosphatase is combined to obtain the sensor. The aptamer sensor has the advantages of magnetism for rapid magnetic separation and rapid and efficient chemiluminescence detection, and has great application value in biomedical detection.