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为从细胞凋亡角度探讨羟丁酸钠、咪达唑仑和异丙酚对心肌缺血/再灌注损伤的防护作用,将72只Wistar大鼠随机分为对照组、羟丁酸钠组、咪达唑仑组和异丙酚组各18只;每组再分为缺血前、缺血30 min和复灌30min3个亚组各6只。分别用透射电镜观察超微结构和用TUNEL法和琼脂糖凝胶电泳测定细胞凋亡。结果表明,与缺血前相比,各组缺血30min及再灌注30min亚组的凋亡指数(AI)均显著增加(P<0.01);与对照组相比,异丙酚缺血30min及再灌注30min亚组的Ai显著降低(P<0.01)。结论:缺血与再灌注组均发生了细胞凋亡,AI均较其缺血前显著增加,且以再灌注组更为明显。从细胞凋亡角度观察,仅异丙酚具有拮抗心肌缺血再灌注损伤的作用。
To investigate the protective effect of sodium oxybate, midazolam and propofol on myocardial ischemia / reperfusion injury from the perspective of apoptosis, 72 Wistar rats were randomly divided into control group, sodium oxybate group, Midazolam group and propofol group, 18 rats in each group. The rats in each group were divided into 3 groups: pre-ischemia, 30 min ischemia and 30 min reperfusion. The ultrastructure was observed by transmission electron microscopy and apoptosis was determined by TUNEL method and agarose gel electrophoresis. The results showed that compared with the pre-ischemic group, the apoptosis index (AI) of the subgroups at 30 min and 30 min after ischemia increased significantly (P <0.01), compared with the control group, Ai in the 30 min reperfusion group decreased significantly (P <0.01). CONCLUSION: Apoptosis occurs in both ischemia and reperfusion groups. AI is significantly increased compared with that before ischemia, and more significantly in reperfusion group. From the perspective of apoptosis, only propofol can antagonize myocardial ischemia-reperfusion injury.