论文部分内容阅读
目的通过检测转化生长因子β1(TGF-β1)及结缔组织生长因子(CTGF)初步探讨应用四环素经纤支镜行微创肺减容术的机制。方法成年杂种犬5只,麻醉后经口插纤支镜入亚段支气管,放入CRE球囊扩张导管,待球囊充起后注入5%四环素混悬液。进行影像学(CT,术后2、4、8、12周)观察及组织病理学(术后12周)观察,逆转录-聚合酶链反应(RT-PCR)及WB法检测减容后局部肺组织TGF-β1及CTGF表达,对侧正常肺组织为对照组。结果术后12周CT检查示5只杂种犬局部肺组织均呈密度增高致密影,未见炎性病变影像,病理检查肉眼见肺表面瘢痕组织形成,切片示阻塞部位失去正常肺组织结构,形成纤维致密结构,RT-PCR及WB结果示TGF-β1及CTGF表达呈阳性,对照组为阴性。结论应用四环素经纤支镜行微创手术可用于肺减容治疗,TGF-β1和CTGF参与了肺减容过程。
Objective To investigate the mechanism of minimally invasive lung volume reduction using tetracycline and bronchofibroscopy through the detection of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF). Methods Five adult mongrel dogs were anesthetized and inserted into the bronchial branch of bronchial artery. The animals were placed in CRE balloon dilatation catheter and infused into 5% tetracycline suspension after filling the balloon. (CT, 2, 4, 8 and 12 weeks postoperatively) and histopathology (12 weeks postoperatively), RT-PCR and WB assay Lung tissue TGF-β1 and CTGF expression, contralateral normal lung tissue as control group. Results CT examination at 12 weeks after operation revealed that the density and density of local lung tissue were increased in all five hybrid dogs. No inflammatory lesions were observed. Pathological examination revealed the formation of scar tissue on the surface of the lung. Slides showed the loss of normal lung tissue structure at the site of obstruction. Fibrous structure, RT-PCR and WB results showed that TGF-β1 and CTGF expression was positive, the control group was negative. Conclusion The application of tetracycline by bronchofiberscopy can be used for lung volume reduction treatment. TGF-β1 and CTGF are involved in lung volume reduction.