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目的:研究漆姑草醇提物(HSJ)对人白血病细胞株HL-60细胞凋亡相关蛋白表达的影响,探讨其诱导凋亡的分子机制。方法:采用不同浓度的HSJ作用HL-60细胞后,Hoechst 33258荧光染色法检测细胞凋亡形态变化;Western blot法检测Caspase-3、Caspase-9、Bcl-2、Bax蛋白的表达。结果:HSJ作用后HL-60细胞出现浓染致密的颗粒状荧光及明显的核形态变化;与阴性对照组相比,凋亡相关蛋白Caspase-3、Caspase-9的表达水平增高,Bcl-2/Bax蛋白的比值降低,差异具有统计学意义(P<0.05)。结论:HSJ能诱导HL-60细胞凋亡,其作用可能与降低Bcl-2/Bax蛋白的比值,增加Caspase-9和Caspase-3蛋白表达水平有关。
OBJECTIVE: To study the effect of ethanol extract of Artemisia japonica (HSJ) on the expression of apoptosis-related proteins in human leukemia cell line HL-60 and to explore its molecular mechanism. Methods: HL-60 cells were treated with different concentrations of HSJ. Hoechst 33258 staining was used to detect the morphological changes of apoptotic cells. The expressions of Caspase-3, Caspase-9, Bcl-2 and Bax were detected by Western blot. Results: HS-treated HL-60 cells showed dense and dense granular fluorescence and obvious nuclear morphological changes. Compared with the negative control group, the expression of Caspase-3 and Caspase-9 were increased, while the expression of Bcl-2 / Bax protein ratio decreased, the difference was statistically significant (P <0.05). CONCLUSION: HSJ can induce the apoptosis of HL-60 cells, which may be related to the decrease of the ratio of Bcl-2 / Bax protein and the increase of Caspase-9 and Caspase-3 protein expression.