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目的:观察急进高原前后正常人单个核细胞中缺氧诱导因子1α(HIF-1α)和核转录因子NF-κB p65亚基(RelA)表达水平的变化,探讨HIF-1α和RelA在急性高原反应(AMS)发生中的作用及机制。方法:对某部赴玉树抗震的士兵分别于进入高原前和进入高原后48h采集外周血,提取单个核细胞中的mRNA和蛋白,采用RT-PCR方法检测RelA和HIF-1αmRNA表达水平,Western blotting法检测HIF-1α蛋白表达水平。Spearman相关分析法分析RelA和HIF-1α基因表达与AMS发生的相关性。结果:与急进高原前比较,进入高原后HIF-1α和RelA mRNA及HIF-1α蛋白表达水平均显著增高(P<0.01);HIF-1αmRNA和RelA mRNA表达呈正相关关系(r=0.806,P<0.01);HIF-1α蛋白表达与AMS的发生率呈正相关关系(r=0.875,P<0.01);HIF-1α和RelA表达不同性别之间比较差异无统计学意义(P>0.05)。结论:HIF-1α蛋白表达水平可作为预测和干预AMS的监测指标。急进高原后HIF-1α和RelA mRNA表达水平显著增加且呈显著正相关关系,提示在其信号传导通路之间存在cross-talk现象。
Objective: To observe the changes of HIF-1α (HIF-1α) and nuclear transcription factor (NF-κB) p65 subunit (RelA) in normal human mononuclear cells before and after reaching the plateau, (AMS) in the role and mechanism. Methods: Some soldiers who went to Yushu earthquake-resistant soldiers were collected before entering the plateau and 48h after entering the plateau to collect the mRNA and protein of mononuclear cells. The mRNA expression of RelA and HIF-1α was detected by RT-PCR. Western blotting Method to detect the expression of HIF-1αprotein. Spearman correlation analysis of RelA and HIF-1α gene expression and the occurrence of AMS. Results: The expression of HIF-1α, RelA mRNA and HIF-1α protein were significantly increased after entering the plateau (P <0.01), and there was a positive correlation between HIF-1α mRNA and RelA mRNA expression (r = 0.806, P < 0.01). There was a positive correlation between the expression of HIF-1α protein and the incidence of AMS (r = 0.875, P <0.01). There was no significant difference in the expression of HIF-1α and RelA between different genders (P> 0.05). Conclusion: The expression of HIF-1α protein can be used as a predictor of AMS. The expression of HIF-1α and RelA mRNA increased significantly and positively correlated with the level of HIF-1α, suggesting a cross-talk phenomenon between their signal transduction pathways.