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用柱状嗜纤维菌 (C .columnaris)、嗜水气单胞菌 (A .hydrophila)和迟缓爱德华氏菌 (E .tarda)等 3种菌中提取的脂多糖 (LPS)作为免疫原 ,分别接种翘嘴鳜后 ,通过测定受免鱼血清中凝集抗体效价、血液中白细胞的吞噬活性和A .hydrophila活菌攻毒的方法 ,比较了接种 3种LPS对翘嘴鳜细菌性败血症的免疫保护力 (RPS)。结果表明 ,与对照组相比 ,受免鱼血清中均可产生较高的凝集及交叉凝集抗体效价 ,血液中白细胞的吞噬活性极显著地升高 ,而且具有较高的交叉RPS。推测LPS对翘嘴鳜既具有特异性免疫活性 ,也存在较强的非特异性免疫活性
The lipopolysaccharide (LPS) extracted from three kinds of bacteria, such as C. columnaris, A. hydrophila and E. tarda, was used as an immunogen and inoculated separately Culter alburnus, the immunological protection of three kinds of LPS against Cephalosporium acutissima bacterial septicemia was compared by measuring the agglutinating antibody titers in the serum-free fish, the phagocytic activity of leukocytes in the blood and the live-inoculation of A. hydrophila Force (RPS). The results showed that, compared with the control group, the titer of agglutination and cross-agglutination antibody could be produced in the serum of immunized fish, the phagocytic activity of leucocytes in the blood increased significantly, and the cross-RPS was higher. It is speculated that LPS has specific immunoconjugate to Cichlid, and also has strong nonspecific immune activity