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采用我国当前流行的小麦条锈菌小种和重要致病类型,在常温条件下对普通小麦-华山新麦草易位系H9015-17进行苗期抗条锈性鉴定,并用当前主要流行小种CYR32对H9015-17与铭贤169的杂交后代及其双亲进行抗条锈性遗传分析,以揭示H9015-17抗条锈性遗传基础。结果显示,H9015-17对小麦条锈菌小种CYR31、CYR32、CYR33和致病类型Su11-4、Su11-7、V26、Su11-11均有良好的抗病性,对当前主要流行小种CYR32的抗病性由1对显性基因控制,暂命名为Yr Hua1。采用分子标记定位技术,筛选到5个与抗病基因Yr Hua1连锁的RGAP标记(M1、M2、M3、M4和M5)和1个SSR标记(Xgwm292),这些标记与抗病基因Yr Hua1的遗传距离分别为17.3、15.7、13.1、3.3、2.9和11.2,并将基因Yr Hua1定位在小麦染色体5DL上。研究结果将为分子标记辅助选择改良小麦抗条锈性提供宝贵的种质材料,建议在抗病育种加以利用。
The current prevalence of wheat stripe rust races and important pathogenic types of common wheat - Huashan New Wheat translocation line H9015-17 at room temperature to identify the stripe rust resistance, and with the current major races CYR32 Stripe rust resistance genetic analysis was carried out on the cross between H9015-17 and Mingxian 169 and their parents to reveal the genetic basis of stripe rust resistance of H9015-17. The results showed that H9015-17 had good disease resistance against wheat stripe rust races CYR31, CYR32, CYR33 and pathogenic types Su11-4, Su11-7, V26, Su11-11. Disease resistance by a pair of dominant genes, temporarily named Yr Hua1. Five marker RGAP markers (M1, M2, M3, M4 and M5) and one SSR marker (Xgwm292) linked to the resistance gene Yr Hua1 were screened by molecular marker technique. These markers were closely related to the inheritance of the resistance gene Yr Hua1 17.3, 15.7, 13.1, 3.3, 2.9 and 11.2 respectively, and the Yr Hua1 gene was located on wheat chromosome 5DL. The results will provide valuable germplasm materials for molecular marker-assisted selection to improve the resistance to stripe rust of wheat, which is suggested to be used in disease-resistant breeding.