以阳离子脂质素作为DNA运载体 ,以肝细胞去唾液酸糖蛋白受体 (ASGPR)的天然配体去唾液酸胎球蛋白 (ASF)作为导向配体 ,用于介导原代大鼠肝细胞基因转移 .结果表明转染复合物中加入ASF可明显提高肝细胞转染率 ,并且明显降低高比例脂质素 DNA复合物转染细胞时对细胞的毒性作用 ,ASGPR的化学合成配体半乳糖基白蛋白在基因转移中亦与ASF具有同样的作用 .因此ASGPR可与阳离子脂质素协同介导肝细胞基因转移 ,为原代肝细胞基因转移提供了一种更为简便、实用、有效的方法 ,有应用于肝基因治疗的前景 . Using cationic lipids as DNA carriers and aspartate fetuinin (ASF), a natural ligand of hepatocyte asialoglycoprotein receptor (ASGPR), as a targeting ligand for mediating primary rat liver Cell gene transfer.The results showed that the addition of ASF to the transfection complex could obviously improve the transfection efficiency of hepatocytes and significantly reduce the cytotoxic effect on the cells transfected with the high proportion of lipofuscin DNA complexes.The chemosynthetic ligand half of ASGPR Lactosylbumin also plays a role in gene transfer as ASF, so ASGPR can cooperate with cationic lipids to mediate the gene transfer of hepatocytes and provide a simple, practical and effective method for primary hepatocyte gene transfer Approach, there is promise for liver gene therapy.