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目的探讨亚砷酸钠对大鼠肝星状细胞(HSC-T6)增殖与凋亡的影响。方法取处于对数生长期的HSC-T6细胞,调整细胞密度为4×10~4/ml,分别暴露于含终浓度为0(对照)、5、15、25μmol/L亚砷酸钠的DMEM高糖培养基24、48、72 h。采用MTT比色法检测细胞增殖活性;采用流式细胞术检测细胞早期凋亡率。结果随染毒剂量和时间增加,5μmol/L组的细胞增殖活性高于对照组,差异有统计学意义(P<0.05),25μmol/L组的细胞增殖活性低于对照组,差异有统计学意义(P<0.05)。HSC-T6细胞形态由染毒前的椭圆形和不规则形转变为染毒后的索条形,有纤维化倾向。随着染毒时间延长,染毒剂量升高,HSC-T6细胞早期凋亡率随之增高,差异有统计学意义(P<0.05),具有剂量-效应和时间-效应关系。结论低剂量亚砷酸钠暴露促进肝星状细胞增殖,高剂量亚砷酸钠暴露抑制肝星状细胞增殖,会改变细胞形态,促进细胞凋亡,具有明显的细胞毒性。
Objective To investigate the effects of sodium arsenite on the proliferation and apoptosis of rat hepatic stellate cells (HSC-T6). Methods HSC-T6 cells were treated with logarithmic growth phase and the cell density was adjusted to 4 × 10-4 / ml. The cells were exposed to DMEM with a concentration of 0 (control), 5, 15, 25μmol / L sodium arsenite High glucose medium 24,48,72 h. MTT colorimetric assay was used to detect cell proliferation; flow cytometry was used to detect the early apoptosis rate. Results The cell proliferation activity in 5μmol / L group was significantly higher than that in control group (P <0.05), while the cell proliferation activity in 25μmol / L group was lower than that in control group with statistical difference Significance (P <0.05). The morphology of HSC-T6 cells transformed from oval and irregular shape before exposure to the stripe shape after exposure, with a tendency of fibrosis. With the prolongation of exposure time and exposure dose, the early apoptosis rate of HSC-T6 cells increased with a significant difference (P <0.05), with dose-effect and time-effect relationship. Conclusion Exposure to low doses of sodium arsenite can promote the proliferation of hepatic stellate cells. Exposure to high doses of sodium arsenite inhibits the proliferation of hepatic stellate cells, altering the cell morphology and promoting apoptosis, with obvious cytotoxicity.