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目的：研究阿霉素诱导的细胞凋亡，探讨人膀胱癌多重抗药性机制。方法：用噻唑蓝（ＭＴＴ）法、光镜及电镜、ＤＮＡ凝胶电泳和原位ＤＮＡ末端转移酶标记法，分别对耐药细胞株ＢＩＵ－８７／ＡＤＭ及敏感细胞株ＢＩＵ－８７细胞进行了阿霉素诱导的细胞凋亡研究。结果：ＢＩＵ－８７／ＡＤＭ细胞存活率明显高于ＢＩＵ－８７细胞，光镜及电镜观察到凋亡细胞形态学改变，凋亡细胞ＤＮＡ凝胶电泳出现ＤＮＡ断片，但并无典型的梯形（Ｌａｄ－ｄｅｒ）改变，ＢＩＵ－８７细胞原位ＤＮＡ末端转移酶标记阳性率高于ＢＩＵ－８７／ＡＤＭ细胞。结论：①阿霉素可诱导人膀胱癌细胞凋亡，而且具有明显的剂量和时间效应。②在相同条件下，与敏感细胞株ＢＩＵ－８７相比，耐药细胞株ＢＩＵ－８７／ＡＤＭ中细胞凋亡明显受到抑制。③细胞凋亡对人膀胱癌多重抗药性的产生具有重要的意义
Objective: To investigate the apoptosis induced by adriamycin and explore the mechanism of multidrug resistance in human bladder cancer. METHODS: MTT assays, light and electron microscopy, DNA gel electrophoresis, and in situ DNA telomerase labeling were performed on drug-resistant cell lines BIU-87/ADM and BIU-87 cells, respectively. Adriamycin-induced apoptosis. RESULTS: The survival rate of BIU-87/ADM cells was significantly higher than that of BIU-87 cells. Morphological changes of apoptotic cells were observed by light and electron microscopy. DNA fragments appeared on DNA gel electrophoresis of apoptotic cells but there were no typical trapezoids (Lad). - der) change, the positive rate of in situ DNA terminal transferase labeling in BIU-87 cells was higher than that in BIU-87/ADM cells. Conclusion: 1 Doxorubicin can induce apoptosis of human bladder cancer cells, and has obvious dose and time effects. 2 Under the same conditions, compared with the sensitive cell line BIU-87, the apoptosis of the drug-resistant cell line BIU-87/ADM was significantly inhibited. 3 Apoptosis plays an important role in the development of multidrug resistance in human bladder cancer