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目的:建立固相微萃取-气相色谱质谱方法,对采自4省市5个地区药用植物绞股蓝的挥发性化学成分进行定性定量分析。方法:采用固相微萃取-气相色谱-质谱(SPME-GC-MS)联用技术对绞股蓝挥发性成分进行分析,利用峰面积归一化法测定各个成分的相对百分含量。结果:5个地区的绞股蓝中,总共分离出67个成分,主要含有醛类、酮类、烷烃类、烯烃类、芳香烃类、醇类和脂类。5个样品中共有成分6个,分别为:3,7-二甲基-1,6-辛二烯-3-醇、萘、α-紫罗酮、香叶基丙酮、β-紫罗酮和6,10,14-三甲基-2-十五烷酮。其中平利绞股蓝中含量最高的成分为3-辛酮(14.93%),宁陕鱼洞村绞股蓝中含量最高的为香叶基丙酮(10.8%),湖南麻姑仙境绞股蓝中含量最高的为3,7-二甲基-1,6-辛二烯-3-醇(22.4%),重庆缙云山和四川青城山绞股蓝中含量最高的均为安息香醛,含量分别为63.16%和38.52%。结论:5个地区绞股蓝中挥发性成分差异性很大。
OBJECTIVE: To establish a solid-phase microextraction-gas chromatography-mass spectrometry (GC-MS / MS) method for the qualitative and quantitative analysis of volatile chemical constituents of medicinal plant Gynostemma pentaphylla collected from 5 provinces and cities. Methods: The volatile components of Gynostemma pentaphyllum were analyzed by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), and the relative percentage of each component was determined by peak area normalization. Results: A total of 67 components were isolated from Gynostemma pentaphyllum in five regions, mainly containing aldehydes, ketones, alkanes, olefins, aromatic hydrocarbons, alcohols and lipids. The five samples shared 6 ingredients, namely 3,7-dimethyl-1,6-octadien-3-ol, naphthalene, α-ionone, geranyl acetone, And 6,10,14-trimethyl-2-pentadecanone. Among them, the content of Pinnacle blue is the highest, which is 3-octanone (14.93%). The highest content of Gynostemma pentaphyllum in Ning-shan Yudong Village is geranyl acetone (10.8% , 7-dimethyl-1, 6-octadien-3-ol (22.4%). The highest contents were benzoin aldehyde in Chongqing Jinyun Mountain and Sichuan Qingcheng Mountain Gynostemma, with contents of 63.16% and 38.52%, respectively. Conclusion: The differences of volatile components in Gynostemma are significant.