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目的 用放射免疫法 (RIA ,radioimmunoassay)和逆转录多聚酶链反应 (RT -PCR ,reversetranscription -polymerasechainreaction)技术 ,分别检测大肠癌患者外周血中的癌胚抗原 (CEA ,carcino -embryonicantigen)和CEAmRNA ,比较两种技术的敏感性。材料和方法 采用RIA和RT -PCR技术分别检测 44例大肠癌患者外周血的CEA和CEAmRNA ,肿瘤按手术标本浸润深度分壁内型和壁外型。壁内型 :14例 ,壁外型 :30例。结果 1 用RT -RCR技术可从 1× 10 7个正常血液单核细胞中检出 10 0个肿瘤细胞 ,即可从大约 5ml血液中检出低至 10 0个LoVo细胞产生的CEAmRNA ;2 CEA和CEAmRNA的阳性表达率分别为 31 8% (14 /44 )和 6 5 9% (2 9/44 ) ,二者存在显著性差异 ,RT -PCR与RIA检测CEA的符合率为 78 6 % (14例RIA检测阳性的病例中有 11例RT -PCR阳性 ) ;3 CEAmRNA在壁内型和壁外型的表达率分别为 35 7% (5 /14 )和 80 % (2 4/30 ) ,两型的表达率存在显著性差异 ;而CEA在壁内型和壁外型的表达率分别为 35 7% (5 /14 )和 30 % (9/30 ) ,两型的表达率无显著性差异。结论 1 RT -PCR技术检测大肠癌患者外周血中的CEAmRNA较RIA检测CEA具有较高的敏感性。2 CEAmRNA的检出率随病程的进展而升高 ,而CEA与病程无明显的相关性
Objective To detect the CEA (carcinoembryonic antigen) and CEA mRNA in peripheral blood of patients with colorectal cancer by radioimmunoassay (RIA) and reverse transcripase-polymerase chain reaction (RT-PCR) Sensitivity of both technologies. Materials and Methods CEA and CEA mRNA were detected in 44 patients with colorectal cancer using RIA and RT-PCR techniques respectively. Wall type: 14 cases, wall appearance: 30 cases. Results 1 Using RT-RTCR technique, 10 0 tumor cells were detected from 1 × 10 7 normal blood mononuclear cells, and as few as 10 0 LoVo cells can be detected from about 5 ml of blood. 2 CEA And CEA mRNA were 31 8% (14/44) and 65 59% (29/44), respectively. There was a significant difference between them, and the coincidence rate of RT-PCR and RIA in CEA was 78 6% ( RT-PCR was positive in 11 out of 14 RIA positive cases); 3 CEA mRNA expression was 35 7% (5/14) and 80% (2 4/30) in the wall and wall, respectively There were significant differences in the expression rates of CEA and CEA between the two groups (35.7% (5/14) and 30% (9/30), respectively) difference. Conclusion 1 RT-PCR detection of peripheral blood in patients with colorectal cancer CEA mRNA RIA detection of CEA has a higher sensitivity. 2 The detection rate of CEAmRNA increased with the progression of the disease, while there was no significant correlation between CEA and the course of disease