采用热酚法从登革２ 型病毒４３ 株（Ｄ２４３） 感染的Ｃ６／３６ 细胞中提取了病毒ＲＮＡ，以病毒ＲＮＡ 为模板，进行Ｄ２４３ 株ＮＳ３ 基因ｃＤＮＡ 片段的反转录ＰＣＲ 扩增，片段长度为１１７６ ｂｐ。将扩增的ｃＤＮＡ 片段克隆到Ｔ 载体ｐＢｌｕｅｓｃｒｉｐｔｋｓⅡ（ ＋ ） 中。通过双脱氧法测定了ｃＤＮＡ片段序列，与国际标准株ＮＧＣ株序列一致。 The viral RNA was extracted from C6 / 36 cells infected with dengue virus type 2 (D243) by hot phenolic method. The viral RNA was used as a template to reverse transcribed PCR of D243 NS3 cDNA fragments Amplified, fragment length of 1176 bp. The amplified cDNA fragment was cloned into the T vector pBluescript ks (+). The cDNA fragment was determined by dideoxy method, which was consistent with the sequence of the international standard strain NGC.