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The discovery of an extraordinarily superconductive large energy gap in SrTiO3 supported single-layer FeSe films has recently initiated a great deal of research interests in surface-enhanced superconductivity and superconductive ultrathin films fabricated on crystal surfaces. On account of the instability of ultra-thin films in air, it is desirable to perform elec-trical transport measurement in ultra-high vaccum (UHV). Here we review the experimental techniques of in situ electrical transport measurement and their applications on superconductive ultrathin films.