论文部分内容阅读
目的建立℃坤宁片中阿魏酸含量测定的方法。方法采用高效液相色谱法, Phenomenex 色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(20:80);流速1.0 ml/min,柱温30℃,检测波长为322 nm。结果阿魏酸在5.18~103.6μg/ml 范围内,呈良好的线性关系(r=0.9993),回收率为97.6%,RSD 为1.2%。结论本方法快速、准确,方法的稳定性、重复性良好,可作为℃坤宁片质量分析控制方法。“,”Objective To establish yi kun ning of ferulic acid content determination method . Methods Using high performance liquid chromatography (HPLC) method, Phenomenex chromatographic column (250 mm x 4.6 mm, 5μm), mobile phase of acetonitrile - 0.1% phosphoric (20:80),Flow rate 1.0 ml min - 1, column temperature 30 ℃, detection wavelength of 322 nm. Results Puerarin in sample quantity is 5.18 μg/ml~103.6μg/ml range , Show a good linear relationship (r = 0.9993),The recovery was 97.6%, RSD was 1.2% . Conclusion This method is rapid and accurate. The stability of the method, good repeatability,and can be used for yi kun ning slice quality control are analyzed.