医用仪器和环境表面的消毒,是预防病毒性乙型肝炎的主要问题之一。尤其是精密仪器的消毒,如纤维光学内窥镜,问题更特殊。这类器械很可能是传播乙型肝炎病毒的媒介。过去提出过用液体消毒剂,但受透入能力之限,效果不能发挥。据此,作者研究了甲醛蒸汽对乙型肝炎表面抗原(HB_sAg)和细菌芽胞的灭活效果。他们模拟自然的环境表面的污染条件,用HB_sAg阳性血清(共4份,2份adw亚型、2份ayw亚型,每份0.25ml)和枯草杆菌芽胞悬液,分别涂布在平皿表面(每份3块),任其干燥。然后放入盛有纤维光学腹腔镜的带塞塑料容器(容量为5.71),每份标本用2块平皿,一块加盖一块不加,其 Disinfection of medical instruments and environmental surfaces is one of the major issues in preventing viral hepatitis B infection. In particular, the disinfection of precision instruments, such as fiber optic endoscopes, the problem is even more special. Such devices are likely to be the vehicle for transmitting hepatitis B virus. In the past proposed using liquid disinfectant, but limited by the penetration capacity, the effect can not play. Accordingly, the author studied the formaldehyde steam inactivation of hepatitis B surface antigen (HB_sAg) and bacterial spores. They mimic natural environmental contamination conditions by applying HBsAg-positive serum (4 copies, 2 copies of adw subtype, 2 copies of ayw subtype, 0.25 ml each) and B. subtilis spores suspension to the plate surface ( Each 3), let it dry. Then placed in a fiber optic laparoscopic plastic stopper with a plug (capacity of 5.71), each specimen with two plates, a piece of a capped without, its