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目的观察自发性Ⅱ型糖尿病OLETF大鼠糖尿病模型出现的时间,罗格列酮对过氧化物酶体增殖体激活受体(PPAR)γ基因表达的干预作用。方法OLETF大鼠随机分成糖尿病对照组和罗格列酮干预组,8周龄时,干预组以罗格列酮每日3mg/kg体重灌胃,直至40周龄。行口服葡萄糖耐量试验(OGTT),鉴定糖尿病发病情况。应用TaqMan实时荧光定量逆转录聚合酶链反应(RTPCR)技术分析PPARγmRNA在糖尿病大鼠各组织中的表达水平及罗格列酮对PPARγ基因的影响。结果至40周龄,对照组糖尿病累积发病率92.5%,糖耐量异常发生率7.5%。干预组糖尿病的累计发病率仅为28.6%,糖耐量异常发生率7.5%,显著低于对照组(P<0.01)。实时荧光定量结果表明:PPARγ在大鼠各组织中均有分布,对照组大鼠各组织中以脂肪表达量最高2.71±0.14(单位:1010拷贝数/100mg组织),是其他组织的10～100倍;其次为血管、胰腺、肾脏、肺脏,分别为1.15±0.10、2.58±0.064、1.52±0.12、4.67±0.088(单位:109拷贝数/100mg组织);心、肝、脾、肌肉中的拷贝数最低分别为7.77±0.11、4.31±0.12、1.51±0.21、2.70±0.087(单位:108拷贝数/100mg组织);干预组各组织中PPARγmRNA的表达量均比对照组高,且在血管、胰腺、肌肉、肾脏组织中差异有统计学意义(P<0.01)。结论成功建立了以OLE
Objective To observe the onset time of diabetes mellitus (OLETF) in spontaneous type 2 diabetes mellitus and the effect of rosiglitazone on the expression of peroxisome proliferator activated receptor (PPAR) γ gene. Methods OLETF rats were randomly divided into diabetic control group and rosiglitazone intervention group. At 8 weeks of age, the intervention group was fed with rosiglitazone 3 mg / kg body weight daily until 40 weeks of age. Oral glucose tolerance test (OGTT) to identify the incidence of diabetes. The expression of PPARγ mRNA in various tissues of diabetic rats and the effect of rosiglitazone on PPARγ gene were analyzed by TaqMan real-time quantitative reverse transcription-polymerase chain reaction (RTPCR). Results At 40 weeks of age, the cumulative incidence of diabetes in the control group was 92.5% and the incidence of impaired glucose tolerance was 7.5%. The cumulative incidence of diabetes in the intervention group was only 28.6%, and the incidence of impaired glucose tolerance was 7.5%, which was significantly lower than that in the control group (P <0.01). The result of real-time fluorescence quantitative analysis showed that PPARγ was distributed in all tissues of rats, and the highest expression of fat was 2.71 ± 0.14 (unit: 1010 copies / 100mg) in other tissues of the control group, which was 10-100 Times; followed by blood vessels, pancreas, kidney and lungs, which were 1.15 ± 0.10,2.58 ± 0.064,1.52 ± 0.12,4.67 ± 0.088 (unit: 109 copies / 100mg tissue); heart, liver, spleen and muscle The lowest were 7.77 ± 0.11,4.31 ± 0.12,1.51 ± 0.21,2.70 ± 0.087 (unit: 108 copies / 100mg tissue). The expression of PPARγmRNA in each group was higher than that in control group , Muscle, kidney tissue difference was statistically significant (P <0.01). Conclusion The successful establishment of OLE