论文部分内容阅读
目的：研制载阿霉素(ADM)的聚乳酸-羟基乙酸共聚物(PLGA)纳米微球的纳米羟基磷灰石/胶原复合支架（ADM-PLGA-NHAC），研究其性质及体外释药特点，探讨其体外抑制人骨肉瘤MG63细胞的作用，为骨肉瘤的治疗提供新策略。方法以纳米羟基磷灰石及胶原为原料制备纳米羟基磷灰石/胶原支架并在其中加载ADM-PLGA纳米微球,通过扫描电子显微镜、体外释放行为等手段评价载药支架材料的性能。以CCK8法、活-死染色评价该复合支架的浸提液在体外对人骨肉瘤MG63细胞株的抗肿瘤活性。结果复合支架的孔径多在100~200μm，孔隙率约为82%，微球与支架间结合较为紧密。复合支架具有良好的缓释特性，28 d内能持续缓慢释放阿霉素。复合支架的浸提液对骨肉瘤MG63细胞生长有明显的抑制作用。结论制备的ADM-PLGA-NHAC复合支架具有良好的药物缓释特性及抗肿瘤效应，是一种具有良好应用前景的抗骨肉瘤骨修复材料。“,”Objective To study preparation and characteristics of nano-hydroxyapatite/collagen scaffolds incorporating ADM-PLGA microspheres(ADM-PLGA-NHAC) and observe its in-vitro anti-tumor effect. Method ADM-PLGA-NHAC were prepared by freezing and lyophylization of composite solution. The characteristic and morphology of the scaffolds were observed by scanning electron microscope, and the release behavior of ADM was investigated in vitro. The antitumor activities of ADM-PLGA-NHAC against human MG63 cell lines were detected by CCK8 colorimetric assay and live/dead cell staining. Result The ADM-PLGA microspheres couldbe uniformly distributed in nano-hydroxyapatite/collagen scaffolds to form ADM-PLGA-NHAC scaffolds, which had 100-200 μm pore diameter and over 82% porosity. The release study indicated that the ADM-PLGA-NHAC show sustained release behavior in vitro. The leaching liquor of ADM-PLGA-NHAC could inhibit MG63 cell lines proliferation in a dose-dependent manner. Conclusion The ADM-PLGA-NHAC shows sustained release characteristics and anti-tumor effect, and it will have a wide application prospect in the fields of osteosarcoma.