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目的:通过动物实验探讨传输靶向COX-2siRNA联合化疗药物对大鼠胃癌细胞生长的抑制作用。方法:24只健康SD大鼠平分为三组,治疗组用COX-2-siRNA转染的胃癌SGC7901细胞接种,同时进行环磷酰胺、丝裂霉素C化疗治疗;阴性对照组,用阴性对照siRNA转染的胃癌SGC7901细胞接种,同时进行环磷酰胺、丝裂霉素C化疗治疗;对照组(n=8),用未经转染的胃癌SGC7901细胞接种,不进行化疗治疗;三组转染后都接种了裸鼠。结果:治疗组、阴性对照组及对照组胃癌细胞凋亡率分别为(22.28±0.12)%、(1.23±0.17)%和(1.03±0.14)%,治疗组与阴性对照组和对照组比较差异都有统计学意义(t=18.152,17.555,P<0.05)。治疗组的抑瘤率为76.7%,阴性对照组和对照组分别为12.8%和6.89%,治疗组的抑瘤率明显高于其他两组(x2=15.211,13.899,P<0.05)。Western blotting检测结果显示治疗组的COX-2蛋白表达含量得到了明显抑制。结论:传输靶向COX-2siRNA和化疗药物的配合应用可有效抑制COX-2蛋白的表达,从而抑制胃癌细胞的生长,从而起到更好的治疗效果。
OBJECTIVE: To investigate the inhibitory effect of COX-2 siRNA combined with chemotherapeutic agents on the growth of gastric cancer cells in rats through animal experiments. Methods: Twenty-four healthy SD rats were equally divided into three groups. The treatment group was inoculated with gastric cancer SGC7901 cells transfected with COX-2-siRNA and treated with cyclophosphamide and mitomycin C chemotherapy. The negative control group was treated with negative control The gastric cancer SGC7901 cells transfected with siRNA were inoculated simultaneously with cyclophosphamide and mitomycin C chemotherapy. The control group (n = 8) was inoculated with non-transfected gastric cancer SGC7901 cells without chemotherapy. Dyed nude mice were vaccinated. Results: The apoptosis rates of gastric cancer cells in treatment group, negative control group and control group were (22.28 ± 0.12)%, (1.23 ± 0.17)% and (1.03 ± 0.14)%, respectively. The difference between treatment group and negative control group and control group All were statistically significant (t = 18.152,17.555, P <0.05). The tumor inhibition rate of the treatment group was 76.7%, the negative control group and the control group were 12.8% and 6.89%, the inhibition rate of the treatment group was significantly higher than the other two groups (x2 = 15.211,13.899, P <0.05). Western blotting results showed that COX-2 protein expression in the treatment group was significantly inhibited. CONCLUSIONS: The combination of COX-2 siRNA and chemotherapeutic drugs can effectively inhibit the expression of COX-2 protein and thus inhibit the growth of gastric cancer cells, thus playing a better therapeutic effect.