目的　观察NGF GDNF基因修饰神经干细胞 (NSC)单独和联合移植对AD模型鼠的学习记忆改善作用。　方法　单侧切断SD大鼠穹窿海马伞 (FF)制备AD模型鼠。术后 8～ 10d ,侧脑室移植基因修饰及未修饰的NSC。移植后 2周 ,进行Morris水迷宫行为学检测。　结果　NGF组和NGF +GDNF组的后 3个时间段的平均逃避潜伏期较损伤组和NSC组明显缩短 (P <0 0 1) ;平台象限游泳距离百分比明显增高 (P <0 0 1) ;GDNF组的平台象限游泳距离百分比高于NSC组 (P <0 0 1) ,但平均逃避潜伏期与NSC组无明显差异。　结论　NGF GDNF基因修饰NSC单独和联合移植对AD模型鼠的行为学有不同程度的疗效 ,其中NGF组和NGF +GDNF组疗效较好 ,但后者并未优于前者 ,GDNF组疗效较差 Objective To observe the effects of NGF GDNF gene modified neural stem cells (NSCs) alone or in combination on the learning and memory of AD mice. Methods Unilateral SD rat fimbria fornix (FF) was prepared AD model rats. 8 to 10 days after transplantation, lateral ventricle genetically modified and unmodified NSC. Two weeks after transplantation, Morris water maze test was performed. Results The average escape latency of the NGF group and the NGF + GDNF group was significantly shorter than that of the injury group and the NSC group (P <0.01), the swimming distance percentage of the platform quadrant was significantly increased (P <0.01), GDNF The swimming distance of the platform quadrants was higher than that of the NSC group (P <0.01), but the mean escape latency was not significantly different from that of the NSC group. Conclusion NGF GDNF gene-modified NSCs alone and in combination have some effects on the behavior of AD model rats. NGF group and NGF + GDNF group are better than the former, while the latter group is not superior to the former, while GDNF group is less effective