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目的探讨SLE患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)孕激素核及膜受体(n PR;m PR)表达情况以及孕激素对IL-2,IL-10的表达调节作用。方法免疫组织化学法及直接免疫荧光法检测48例SLE患者及26例健康女性PBMCs n PR及m PR表达;体外培养SLE患者n PR和/或m PR表达阳性的PBMCs,以不同浓度孕激素(1×10-8mol/L,1×10-7mol/L,1×10-6mol/L)进行诱导,ELISA法检测培养液IL-2,IL-10表达。结果 n PR+和/或m PR+PBMCs阳性率SLE组(70.83%)高于健康组(42.31%);SLE活动期(90.00%)高于稳定期(57.14%);差异均有统计学意义(P均<0.05)。孕激素浓度≥1×10-7mol/L时,上调n PR+PBMCs的IL-10表达;孕激素浓度为1×10-6mol/L时,上调m PR+PBMCs的IL-10表达,而下调n PR+PBMCs的IL-2表达(P均<0.05)。结论 SLE患者PBMCs存在孕激素核及膜受体表达异常。高浓度孕激素可通过受体依赖机制上调PBMCs IL-10表达,下调IL-2表达。
Objective To investigate the expression of progesterone nucleus and membrane receptor (n PR; m PR) in peripheral blood mononuclear cells (PBMCs) in SLE patients and the effect of progesterone on the expression of IL-2 and IL-10. Methods The expression of n PR and m PR in 48 SLE patients and 26 healthy women PBMCs were detected by immunohistochemistry and direct immunofluorescence. PBMCs positive for n PR and / or m PR expression in SLE patients were cultured in vitro with different concentrations of progesterone 1 × 10-8mol / L, 1 × 10-7mol / L, 1 × 10-6mol / L). The expression of IL-2 and IL-10 in culture medium was detected by ELISA. Results The positive rate of n PR + and / or m PR + PBMCs in SLE group (70.83%) was higher than that in healthy group (42.31%), the activity of SLE was higher than that in stable phase (57.14%), the difference was statistically significant P <0.05). The progesterone concentration of 1 × 10-7mol / L, the n PR + PBMCs IL-10 expression; progesterone concentration of 1 × 10-6mol / L, upregulation of m PR PBMCs IL-10 expression, and down n PR + PBMCs (all P <0.05). Conclusion The expression of progesterone receptor and membrane receptor in PBMCs of patients with SLE is abnormal. High concentrations of progesterone can up-regulate IL-10 expression and down-regulate IL-2 expression in PBMCs through receptor-dependent mechanism.